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LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway

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Abstract

Keloid is a skin disease characterized by fibrous hyperplasia, which is often difficult to cure. Long non-coding RNAs (lncRNAs) have been shown to be associated with the development of many diseases. However, the role and mechanism of lncRNA H19 in keloid has been less studied. Our study found that lncRNA H19 expression was increased in keloid tissues and fibroblasts. Besides, H19 knockdown hindered the proliferation, migration, invasion, extracellular matrix (ECM) deposition, and enhanced the apoptosis of keloid fibroblasts. Further experiments showed that microRNA (miR)-769-5p could be sponged by H19, and its knockdown reversed the suppression effect of H19 knockdown on keloid formation. Eukaryotic initiation factor 3A (EIF3A) was found to be a target of miR-769-5p, and its overexpression inverted the inhibition effect of miR-769-5p overexpression on keloid formation. Moreover, the expression of EIF3A was regulated by H19 and miR-769-5p in keloid fibroblasts. Collectively, LncRNA H19 might play an active role in keloid formation, which might provide a new target for the treatment of keloid.

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Authors and Affiliations

  1. Department of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

    Lingang Xu, Nan Sun, Guangshuai Li & Linbo Liu

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  1. Lingang Xu
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  2. Nan Sun
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  3. Guangshuai Li
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  4. Linbo Liu
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All authors made substantial contribution to conception and design, acquisition of the data, or analysis and interpretation of the data; take part in drafting the article or revising it critically for important intellectual content; gave final approval of the revision to be published; and agree to be accountable for all aspect of the work.

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Correspondence to Linbo Liu.

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The present study was approved by the ethical review committee of The First Affiliated Hospital of Zhengzhou University. Written informed consent was obtained from all enrolled patients.

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Xu, L., Sun, N., Li, G. et al. LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway. Mol Cell Biochem 476, 1477–1487 (2021). https://doi.org/10.1007/s11010-020-04024-x

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  • DOI: https://doi.org/10.1007/s11010-020-04024-x

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