LINC00324 affects non-small cell lung cancer cell proliferation and invasion through regulation of the miR-139-5p/IGF1R axis

Abstract

Long non-coding RNAs (lncRNAs) are proved to perform critical function in regulating cancer cell behavior. It is reported that LINC00324 promotes lung adenocarcinoma development by regulating miR-615-5p/AKT1 axis. This study aimed to demonstrate whether LINC00324 participates in non-small cell lung cancer (NSCLC) pathogenesis through other molecular mechanism. Relative mRNA, lncRNA, and microRNA levels were analyzed using quantitative real-time–polymerase chain reaction (qRT-PCR). Western blot was used to detect protein level. MTT assay shown proliferation ability and transwell assay shown invasive ability. Luciferase reporter assay illustrated the interaction between RNA molecules. In NSCLC, the high expression of LINC00324 had correlation with the poor prognosis. LINC00324 promoted the proliferation and invasion of NSCLC cells while miR-139-5p inhibited these behaviors. LINC00324 overexpression promoted insulin-like growth factor 1 receptor (IGF1R) expression via absorbing miR-139-5p. The tumor-promoting effects of LINC00324 were attenuated through miR-139-5p overexpression. Highly expressed LINC00324 in NSCLC through sponged miR-139-5p to elevate IGF1R expression and promoted cell proliferation and invasion. This research demonstrated that LINC00324 is a potential NSCLC diagnosis and therapy target.

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References

  1. 1.

    Absenger G, Terzic J, Bezan A (2017) ASCO update: lung cancer. Memo 10(4):224–227. https://doi.org/10.1007/s12254-017-0373-x

    PubMed  PubMed Central  Article  Google Scholar 

  2. 2.

    Cheng G, Akhurst T (2018) Lung cancer. PET Clin 13(1):xi–xii. https://doi.org/10.1016/j.cpet.2017.10.001

    PubMed  Article  Google Scholar 

  3. 3.

    Zakaria N, Satar NA, Abu Halim NH, Ngalim SH, Yusoff NM, Lin J, Yahaya BH (2017) Targeting lung cancer stem cells: research and clinical impacts. Front Oncol 7:80. https://doi.org/10.3389/fonc.2017.00080

    PubMed  PubMed Central  Article  Google Scholar 

  4. 4.

    Alghamdi HI, Alshehri AF, Farhat GN (2018) An overview of mortality & predictors of small-cell and non-small cell lung cancer among Saudi patients. J Epidemiol Glob Health 7(Suppl 1):S1–S6. https://doi.org/10.1016/j.jegh.2017.09.004

    PubMed  Article  Google Scholar 

  5. 5.

    Zhou YY, Hu ZG, Zeng FJ, Han J (2016) Clinical profile of cyclooxygenase-2 inhibitors in treating non-small cell lung cancer: a meta-analysis of Nine Randomized Clinical Trials. PLoS ONE 11(3):e0151939. https://doi.org/10.1371/journal.pone.0151939

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  6. 6.

    Quinn JJ, Chang HY (2016) Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet 17(1):47–62. https://doi.org/10.1038/nrg.2015.10

    CAS  PubMed  Article  Google Scholar 

  7. 7.

    Jiang Y, Malouf GG, Zhang J, Zheng X, Chen Y, Thompson EJ, Weinstein JN, Yuan Y, Spano JP, Broaddus R, Tannir NM, Khayat D, Lu KH, Su X (2015) Long non-coding RNA profiling links subgroup classification of endometrioid endometrial carcinomas with trithorax and polycomb complex aberrations. Oncotarget 6(37):39865–39876. https://doi.org/10.18632/oncotarget.5399

    PubMed  PubMed Central  Article  Google Scholar 

  8. 8.

    Guo T, Li J, Zhang L, Hou W, Wang R, Zhang J, Gao P (2019) Multidimensional communication of microRNAs and long non-coding RNAs in lung cancer. J Cancer Res Clin Oncol 145(1):31–48. https://doi.org/10.1007/s00432-018-2767-5

    CAS  PubMed  Article  Google Scholar 

  9. 9.

    Pan ZH, Guo XQ, Shan J, Luo SX (2018) LINC00324 exerts tumor-promoting functions in lung adenocarcinoma via targeting miR-615-5p/AKT1 axis. Eur Rev Med Pharmacol Sci 22(23):8333–8342. https://doi.org/10.26355/eurrev_201812_16531

    PubMed  Article  Google Scholar 

  10. 10.

    Xu W, Hang M, Yuan CY, Wu FL, Chen SB, Xue K (2015) MicroRNA-139-5p inhibits cell proliferation and invasion by targeting insulin-like growth factor 1 receptor in human non-small cell lung cancer. Int J Clin Exp Pathol 8(4):3864–3870

    CAS  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Schwartz AG, Cote ML (2016) Epidemiology of Lung Cancer. Adv Exp Med Biol 893:21–41. https://doi.org/10.1007/978-3-319-24223-1_2

    PubMed  Article  Google Scholar 

  12. 12.

    Alberg AJ, Brock MV, Ford JG, Samet JM, Spivack SD (2013) Epidemiology of lung cancer: Diagnosis and management of lung cancer, 3rd ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest 143(5 Suppl):e1S–e29S. https://doi.org/10.1378/chest.12-2345

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  13. 13.

    Nanavaty P, Alvarez MS, Alberts WM (2014) Lung cancer screening: advantages, controversies, and applications. Cancer Control 21(1):9–14. https://doi.org/10.1177/107327481402100102

    PubMed  Article  Google Scholar 

  14. 14.

    Wang KC, Chang HY (2011) Molecular mechanisms of long noncoding RNAs. Mol Cell 43(6):904–914. https://doi.org/10.1016/j.molcel.2011.08.018

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  15. 15.

    Zhang H, Chen Z, Wang X, Huang Z, He Z, Chen Y (2013) Long non-coding RNA: a new player in cancer. J Hematol Oncol 6:37. https://doi.org/10.1186/1756-8722-6-37

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  16. 16.

    White NM, Cabanski CR, Silva-Fisher JM, Dang HX, Govindan R, Maher CA (2014) Transcriptome sequencing reveals altered long intergenic non-coding RNAs in lung cancer. Genome Biol 15(8):429. https://doi.org/10.1186/s13059-014-0429-8

    PubMed  PubMed Central  Article  Google Scholar 

  17. 17.

    Loewen G, Jayawickramarajah J, Zhuo Y, Shan B (2014) Functions of lncRNA HOTAIR in lung cancer. J Hematol Oncol 7:90. https://doi.org/10.1186/s13045-014-0090-4

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  18. 18.

    Zou Z, Ma T, He X, Zhou J, Ma H, Xie M, Liu Y, Lu D, Di S, Zhang Z (2018) Long intergenic non-coding RNA 00324 promotes gastric cancer cell proliferation via binding with HuR and stabilizing FAM83B expression. Cell Death Dis 9(7):717. https://doi.org/10.1038/s41419-018-0758-8

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  19. 19.

    Li S, Gao M, Li Z, Song L, Gao X, Han J, Wang F, Chen Y, Li W, Yang J, Han X (2016) Role of microRNAs in metastasis of non-small cell lung cancer. Front Biosci (Landmark Ed) 21:998–1005

    CAS  Article  Google Scholar 

  20. 20.

    Militello G, Weirick T, John D, Doring C, Dimmeler S, Uchida S (2017) Screening and validation of lncRNAs and circRNAs as miRNA sponges. Brief Bioinform 18(5):780–788. https://doi.org/10.1093/bib/bbw053

    CAS  PubMed  Article  Google Scholar 

  21. 21.

    Riedemann J, Macaulay VM (2006) IGF1R signalling and its inhibition. Endocr Relat Cancer 13(Suppl 1):S33–43. https://doi.org/10.1677/erc.1.01280

    CAS  PubMed  Article  Google Scholar 

  22. 22.

    Hartog H, Wesseling J, Boezen HM, van der Graaf WT (2007) The insulin-like growth factor 1 receptor in cancer: old focus, new future. Eur J Cancer 43(13):1895–1904. https://doi.org/10.1016/j.ejca.2007.05.021

    CAS  PubMed  Article  Google Scholar 

  23. 23.

    Larsson O, Girnita A, Girnita L (2005) Role of insulin-like growth factor 1 receptor signalling in cancer. Br J Cancer 92(12):2097–2101. https://doi.org/10.1038/sj.bjc.6602627

    CAS  PubMed  PubMed Central  Article  Google Scholar 

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Correspondence to Congwen Zhuang.

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This experiment was approved by Ethics Committee of the Fujian Cancer Hospital & Fujian Medical University Cancer Hospital.

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Zhang, M., Lin, B., Liu, Y. et al. LINC00324 affects non-small cell lung cancer cell proliferation and invasion through regulation of the miR-139-5p/IGF1R axis. Mol Cell Biochem 473, 193–202 (2020). https://doi.org/10.1007/s11010-020-03819-2

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Keywords

  • LINC00324
  • miR-139-5p
  • IGF1R
  • NSCLC