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Effect of exercise on telomere length and telomere proteins expression in mdx mice

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Abstract

In Duchenne muscular dystrophy (DMD), telomere shortening has been postulated to contribute to the failure of regenerative activity promoting the premature senescence of satellite cells. The aim of the present study was to investigate the telomere length and the expression of telomeric repeat-binding factor-1 (TRF1), poly (ADP-ribose) polymerase-1 (PARP1) and mouse telomerase reverse transcriptase (MTERT) in gastrocnemius, tibialis anterior and diaphragm muscles of the murine model of DMD, the mdx mouse and whether a chronic protocol of forced exercise impacts on them. Our results confirmed a telomere shortening in mdx muscles, more evident in the diaphragm, in which exercise induced a greater shortening than in wild-type mice. Moreover, we showed for the first time in mdx an increased TRF1 and PARP1 expression and an augmented activity of MTERT, further enhanced by exercise. These results reinforce the hypothesis that a deregulation of mechanisms involved in telomere length occurs and may pave the way for the test of compounds targeting proteins modulating telomere maintenance as a novel strategy to treat dystrophinopathies.

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Funding

Part of the project has been supported by MIUR PRIN 2015 to ADL [n. 2015MJBEM2_005]. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

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Author notes

  1. Gian Luca Vita and M’hammed Aguennouz have contributed equally to this work

Authors and Affiliations

  1. Nemo Sud Clinical Centre for Neuromuscular Disorders, Messina University Hospital, Via Consolare Valeria 1, 98125, Messina, Italy

    Gian Luca Vita, Maria Sframeli, Giuseppe Vita & Sonia Messina

  2. Department of Clinical and Experimental Medicine, University of Messina, Via Consolare Valeria 1, 98125, Messina, Italy

    M’hammed Aguennouz, Rosaria Oteri, Francesca Polito, Norma Licata, Sara Romeo, Maria Grazia Distefano, Matteo La Rosa, Carmen Bonanno, Giulia Nicocia, Giuseppe Vita & Sonia Messina

  3. Mohammed VI University of Health Sciences, Casablanca, Morocco

    M’hammed Aguennouz

  4. Unit of Pharmacology, Department of Pharmacy and Drug Sciences, University of Bari “A. Moro”, Via Orabona 4, 70125, Bari, Italy

    Francesca Sanarica, Paola Mantuano & Annamaria De Luca

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  1. Gian Luca Vita
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Contributions

GLV, MA, GV, ADL and SM contributed to the conception and design of the study. MS, FS, PM, RO, FP, NL and SR performed the experiments and analysed the data. All authors contributed to manuscript draft. All authors approved the final version.

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Correspondence to M’hammed Aguennouz.

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Vita, G.L., Aguennouz, M., Sframeli, M. et al. Effect of exercise on telomere length and telomere proteins expression in mdx mice. Mol Cell Biochem 470, 189–197 (2020). https://doi.org/10.1007/s11010-020-03761-3

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