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CircularRNA-9119 promotes the proliferation of cervical cancer cells by sponging miR-126/MDM4

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Abstract

The aim of this study was to investigate the role of circular RNA-9119 (circ9119) in cervical cancer (CC) and the microRNA-126-3p (miR-126)-based molecular mechanism underlying CC. circ9119 and MDM4 were initially overexpressed, and miR-126 expression was found to be reduced in CC cells and tissues. A series of mimics, inhibitors, overexpressing plasmids or siRNAs were introduced into CC cells to alter the circ9119, miR-126, and MDM4 expressions. Cell-based experiments showed that silencing of circ9119 or the upregulation of miR-126 resulted in suppressed proliferation, accompanied by the induced apoptosis of CC cells. The dual-luciferase reporter assay highlighted that circ9119 functioned as an miR-126 ceRNA to increase MDM4 expression. In vivo experiments further confirmed the suppressed tumor growth caused by circ9119 silencing. Our findings demonstrated that circ9119 acts as an oncogene in CC. Our study provides evidence for targeting circ9119 for the treatment of CC.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Gynecology Second Ward, Linyi Central Hospital, No. 17, Health Road, Yishui County, Linyi, 276400, Shandong, China

    Yonghui Tian, Zonglan Xu & Jingjing Fu

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  1. Yonghui Tian
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  2. Zonglan Xu
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  3. Jingjing Fu
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Correspondence to Yonghui Tian.

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The procedures conducted in this study were approved by the Ethics Committee of Linyi Central Hospital. Animal experiments were performed under the approval of the Animal Ethics Committee of Linyi Central Hospital.

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Tian, Y., Xu, Z. & Fu, J. CircularRNA-9119 promotes the proliferation of cervical cancer cells by sponging miR-126/MDM4. Mol Cell Biochem 470, 53–62 (2020). https://doi.org/10.1007/s11010-020-03745-3

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  • DOI: https://doi.org/10.1007/s11010-020-03745-3

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