Abstract
This study evaluated the cardioprotective effects of a miR-1275 mimic in a rat model of myocardial ischemia–reperfusion (I/R)-induced myocardial injury (MI). Three groups of rats were established: a sham-operated group, a MI group and a MI+miR-1275 group pretreated for 1 week i.p. with a miR-1275 mimic at a concentration of 30 pmol/mL. MI was induced by I/R. The levels of myocardial enzymes in serum were estimated in all rats, together with haemodynamic functions. The effects of the miR-1275 mimic were determined based on the serum concentrations of inflammatory mediators in the treated vs. sham and MI rats. In addition, western blot assay and immunohistochemical analyses were performed to examine the effect of the miR-1275 mimic on the Wnt/NF-kB signalling pathway in MI rats. Treatment with the miR-1275 mimic attenuated the altered levels of myocardial enzymes and haemodynamic functions seen in MI rats. The myocardial infarct was smaller in rats treated with the miR-1275 mimic than in MI rats. The miR-1275 mimic also reduced myocardiocyte apoptosis and ameliorated the altered Wnt/KF-kB pathway. These results demonstrate the efficacy of the miR-1275 mimic in preventing myocardial I/R-induced MI in rats, by regulating the Wnt/NF-κB pathway.
Similar content being viewed by others
Change history
13 September 2022
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s11010-022-04547-5
References
Gaziano T, Reddy KS, Paccaud F, et al. (2006) Cardiovascular disease. In: Jamison DT, Breman JG, Measham AR, et al., editors. Disease control priorities in developing countries Chapt. 33. 2nd edn. Washington (DC): The International Bank for Reconstruction and Development/The World Bank
Kalogeris T, Baines CP, Krenz M, Korthuis RJ (2016) Ischemia/reperfusion. Compr Physiol 7(1):113–170
Wu MY, Yiang GT, Liao WT, Tsai AP, Cheng YL, Cheng PW, Li CY, Li CJ (2018) Current mechanistic concepts in ischemia and reperfusion injury. Cell Physiol Biochem 46(4):1650–1667
Ong SB, Hernández-Reséndiz S, Crespo-Avilan GE et al (2018) Inflammation following acute myocardial infarction: multiple players, dynamic roles, and novel therapeutic opportunities. Pharmacol Ther 186:73–87
Dimitriadis GK, Kaur J, Adya R et al (2018) Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion. Oncotarget 9(24):16678–16690
Bastakoty D, Young PP (2016) Wnt/β-catenin pathway in tissue injury: roles in pathology and therapeutic opportunities for regeneration. FASEB J. 30(10):3271–3284
Ma B, Hottiger MO (2016) Crosstalk between Wnt/β-catenin and NF-κB signaling pathway during inflammation. Front Immunol 7:378
Du Q, Geller DA (2010) Cross-regulation between Wnt and NF-κB signaling pathways. For Immunopathol Dis Therap 1(3):155–181
Ha TY (2011) MicroRNAs in human diseases: from cancer to cardiovascular disease. Immune Netw 11(3):135–154
de Lucia C, Komici K, Borghetti G et al (2017) microRNA in cardiovascular aging and age-related cardiovascular diseases. Front Med (Lausanne) 4:74
Pang L, You L, Ji C, Shi C, Chen L, Yang L, Huang F, Zhou Y, Zhang J, Chen X, Guo X (2016) miR-1275 inhibits adipogenesis via ELK1 and its expression decreases in obese subjects. J Mol Endocrinol 57(1):33–43
Huan T, Rong J, Tanriverdi K, Meng Q, Bhattacharya A, McManus DD, Joehanes R, Assimes TL, McPherson R, Samani NJ, Erdmann J, Schunkert H, Courchesne P, Munson PJ, Johnson AD, O'Donnell CJ, Zhang B, Larson MG, Freedman JE, Levy D, Yang X (2015) Dissecting the roles of microRNAs in coronary heart disease via integrative genomic analyses. Arterioscler Thromb Vasc Biol 35(4):1011–1021
Yu L, Zhang W, Huang C, Liang Q, Bao H, Gong Z, Xu M, Wang Z, Wen M, Cheng X (2018) FoxO4 promotes myocardial ischemia-reperfusion injury: the role of oxidative stress-induced apoptosis. Am J Transl Res 10(9):2890–2900
Mahajan UB, Chandrayan G, Patil CR et al (2017) The protective effect of apigenin on myocardial injury in diabetic rats mediating activation of the PPAR-γ pathway. Int J Mol Sci 18(4):756
Lim KH, Ko D, Kim JH (2013) Cardioprotective potential of Korean red ginseng extract on isoproterenol-induced cardiac injury in rats. J Ginseng Res 37(3):273–282
Zhang XQ, Tang R, Li L et al (2013) Cardiomyocyte-specific p65 NF-κB deletion protects the injured heart by preservation of calcium handling. Am J Physiol Heart Circ Physiol 305(7):H1089–H1097
Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Matsuo T (2006) Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts. Clin Exp Immunol 144(3):494–502
Tian ZQ, Jiang H, Lu ZB (2018) MiR-320 regulates cardiomyocyte apoptosis induced by ischemia-reperfusion injury by targeting AKIP1. Cell Mol Biol Lett 23:41
Chen C, Liu TS, Zhao SC, Yang WZ, Chen ZP, Yan Y (2018) XIAP impairs mitochondrial function during apoptosis by regulating the Bcl-2 family in renal cell carcinoma. Exp Ther Med 15(5):4587–4593
Park HA, Broman K, Stumpf A, Kazyak S, Jonas EA (2018) Nutritional regulators of Bcl-xL in the brain. Molecules 23(11):3019
Cleutjens JPM, Blankesteijn WM, Daemen MJAP, Smits JFM (1999) The infarcted myocardium: simply dead tissue, or a lively target for therapeutic interventions. Cardiovasc Res 44:232–241
Frangogiannis NG (2014) The inflammatory response in myocardial injury, repair and remodeling. Nat Rev Cardiol 11:255–265
Fu WB, Wang WE, Zeng CY (2019) Wnt signaling pathways in myocardial infarction and the therapeutic effects of Wnt pathway inhibitors. Acta Pharmacol Sin 40(1):9–12
Wang Y, Zheng X, Wang Q, Zheng M, Pang L (2017) Activation of Wnt signaling reduces high-glucose mediated damages on skin fibroblast cells. Iran J Basic Med Sci 20(8):944–950
Borne SWMVD, Diez J, Blankesteijn WM, Verjans J, Hofstra L, Narula J (2010) Myocardial remodeling after infarction: the role of myofibroblasts. Nat Rev Cardiol 7:30–37
Daskalopoulos EP, Janssen BJ, Blankesteijn WM (2012) Myofibroblasts in the infarct area: concepts and challenges. Microsc Microanal 18:35–49
Acknowledgements
The authors thank the China–Japan Union Hospital of Jilin University, China, for providing the necessary facility to conduct this study.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s11010-022-04547-5
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Jiang, T., You, H., You, D. et al. RETRACTED ARTICLE: A miR-1275 mimic protects myocardiocyte apoptosis by regulating the Wnt/NF-κB pathway in a rat model of myocardial ischemia–reperfusion-induced myocardial injury. Mol Cell Biochem 466, 129–137 (2020). https://doi.org/10.1007/s11010-020-03695-w
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-020-03695-w