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miR-26 suppresses renal cell cancer via down-regulating coronin-3

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Abstract

Coronin-3 (coronin-1C), a homotrimer F-actin-binding protein, has been reported to be important for metastasis in several types of cancers such as lung cancer, gastric cancer, and breast cancer. Here, we present an investigation of the expression and function of coronin-3 in renal cell cancer for the first time. We also confirmed that miR-26 directly targets coronin-3 and down-regulates its expression by western blot assay and dual-luciferase reporter system. The results of MTT and colony formation assay showed that miR-26 suppressed cell proliferation. Wound healing and transwell assay revealed that miR-26 inhibited migration and invasion of renal cancer cell. Moreover, overexpression of coronin-3 could reverse the miR-26-induced inhibition in cell growth and metastasis. Thus, our study suggests that coronin-3 should serve as a potential therapeutic target in renal cell cancer and provide a candidate for miRNA therapy.

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References

  1. Hsieh JJ, Purdue MP, Signoretti S, Swanton C, Albiges L, Schmidinger M, Heng DY, Larkin J, Ficarra V (2017) Renal cell carcinoma. Nat Rev Dis Primers 3:17009. https://doi.org/10.1038/nrdp.2017.9

    Article  PubMed  PubMed Central  Google Scholar 

  2. Cho E, Adami HO, Lindblad P (2011) Epidemiology of renal cell cancer. Hematol Oncol Clin 25(4):651–665

    Article  Google Scholar 

  3. Barata PCA, Rini BI (2017) Treatment of renal cell carcinoma: current status and future directions. CA: Cancer J Clin 67(6):507–524

    Google Scholar 

  4. Mennitto A, Verzoni E, Grassi P, Ratta R, Fuca G, Procopio G (2017) Multimodal treatment of advanced renal cancer in 2017. Exp Rev Clin Pharmacol. https://doi.org/10.1080/17512433.2017.1386552

    Article  Google Scholar 

  5. de Hostos EL (1999) The coronin family of actin-associated proteins. Trends Cell Biol 9(9):345–350

    Article  Google Scholar 

  6. Uetrecht AC, Bear JE (2006) Coronins: the return of the crown. Trends Cell Biol 16(8):421–426

    Article  CAS  Google Scholar 

  7. Pantaloni D, Le Clainche C, Carlier MF, Carlier MF (2001) Mechanism of actin-based motility. Science 292(5521):1502–1506

    Article  CAS  Google Scholar 

  8. Giese A, Bjerkvig R, Berens ME, Berens ME, Westphal M, Westphal M (2003) Cost of migration: invasion of malignant gliomas and implications for treatment. J Clin Oncol 21(8):1624–1636

    Article  CAS  Google Scholar 

  9. Samarin SN, Koch S, Ivanov AI, Parkos CA, Nusrat A (2010) Coronin 1C negatively regulates cell-matrix adhesion and motility of intestinal epithelial cells. Biochem Biophys Res Commun 391(1):394–400

    Article  CAS  Google Scholar 

  10. Thal D, Xavier CP, Rosentreter A, Linder S, Friedrichs B, Waha A, Pietsch T, Stumpf M, Noegel A, Clemen C (2008) Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy. J Pathol 214(4):415–424

    Article  CAS  Google Scholar 

  11. Sun Y, Shang Y, Ren G, Zhou L, Feng B, Li K, Deng L, Liang J, Lu Y, Wang X (2014) Coronin3 regulates gastric cancer invasion and metastasis by interacting with Arp2. Cancer Biol Ther 15(9):1163–1173. https://doi.org/10.4161/cbt.29501

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Mataki H, Enokida H, Chiyomaru T, Mizuno K, Matsushita R, Goto Y, Nishikawa R, Higashimoto I, Samukawa T, Nakagawa M, Inoue H, Seki N (2014) Downregulation of the microRNA-1/133a cluster enhances cancer cell migration and invasion in lung-squamous cell carcinoma via regulation of coronin1C. J Hum Genet 60:53. https://doi.org/10.1038/jhg.2014.11113

    Article  PubMed  Google Scholar 

  13. Ren G, Tian Q, An Y, Feng B, Lu Y, Liang J, Li K, Shang Y, Nie Y, Wang X, Fan D (2012) Coronin 3 promotes gastric cancer metastasis via the up-regulation of MMP-9 and cathepsin K. Mol Cancer 11(1):67

    Article  CAS  Google Scholar 

  14. Castagnino A, Castro-Castro A, Irondelle M, Guichard A, Lodillinsky C, Fuhrmann L, Vacher S, Agüera-González S, Zagryazhskaya-Masson A, Romao M, El Kesrouani C, Noegel AA, Dubois T, Raposo G, Bear JE, Clemen CS, Vincent-Salomon A, Bièche I, Chavrier P (2018) Coronin 1C promotes triple-negative breast cancer invasiveness through regulation of MT1-MMP traffic and invadopodia function. Oncogene 37(50):6425–6441. https://doi.org/10.1038/s41388-018-0422-x

    Article  CAS  PubMed  Google Scholar 

  15. Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Fujimura L, Yoshino H, Kawakami K, Chiyomaru T, Enokida H, Nakagawa M, Okamoto Y, Seki N (2011) Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma. Int J Oncol 38(1):209–217

    CAS  PubMed  Google Scholar 

  16. Li J, Liang Y, Lv H, Meng H, Xiong G, Guan X, Chen X, Bai Y, Wang K (2017) miR-26a and miR-26b inhibit esophageal squamous cancer cell proliferation through suppression of c-MYC pathway. Gene 625:1–9

    Article  CAS  Google Scholar 

  17. Cai ZG, Wu HB, Xu XP, Li W (2018) Down-regulation of miR-26 plays essential roles in TGFbeta-induced EMT. Cell Biol Int. https://doi.org/10.1002/cbin.11029

    Article  PubMed  Google Scholar 

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Funding

The paper is supported by the Project of Science and Technology Bureau of Xiamen (3502Z20184033) and the Project of Science and Technology Bureau of Xiamen (2019S2949).

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Authors and Affiliations

  1. Department of Urology, Zhongshan Hospital Xiamen University, Xiamen, 361000, Fujian, China

    Xin Jun Wang, Zhi Jian Yan, Guang Cheng Luo, Yi Yan Chen & Pei Ming Bai

  2. Teaching Hospital of Fujian Medical University, Xiamen, 361000, Fujian, China

    Xin Jun Wang, Zhi Jian Yan, Guang Cheng Luo & Pei Ming Bai

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  1. Xin Jun Wang
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  3. Guang Cheng Luo
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Correspondence to Guang Cheng Luo.

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Wang, X.J., Yan, Z.J., Luo, G.C. et al. miR-26 suppresses renal cell cancer via down-regulating coronin-3. Mol Cell Biochem 463, 137–146 (2020). https://doi.org/10.1007/s11010-019-03636-2

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  • DOI: https://doi.org/10.1007/s11010-019-03636-2

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