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RETRACTED ARTICLE: Baicalin augments the differentiation of osteoblasts via enhancement of microRNA-217

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This article was retracted on 29 February 2024

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Abstract

Baicalin (BAI), a sort of flavonoid monomer, acquires from Scutellaria baicalensis Georgi, which was forcefully reported in diversified ailments due to the pleiotropic properties. But, the functions of BAI in osteoblast differentiation have not been addressed. The intentions of this study are to attest the influences of BAI in the differentiation of osteoblasts. MC3T3-E1 cells or rat primary osteoblasts were exposed to BAI, and then cell viability, ALP activity, mineralization process, and Runx2 and Ocn expression were appraised through implementing CCK-8, p-nitrophenyl phosphate (pNPP), Alizarin red staining, western blot, and RT-qPCR assays. The microRNA-217 (miR-217) expression was evaluated in MC3T3-E1 cells or rat primary osteoblasts after BAI disposition; meanwhile, the functions of miR-217 in BAI-administrated MC3T3-E1 cells were estimated after miR-217 inhibitor transfection. The impacts of BAI and miR-217 inhibition on Wnt/β-catenin and MEK/ERK pathways were probed to verify the involvements in BAI-regulated the differentiation of osteoblasts. BAI accelerated cell viability, osteoblast activity, and Runx2 and Ocn expression in MC3T3-E1 cells or rat primary osteoblasts, and the phenomena were mediated via activations of Wnt/β-catenin and MEK/ERK pathways. Elevation of miR-217 was observed in BAI-disposed MC3T3-E1 cells or rat primary osteoblasts, and miR-217 repression annulled the functions of BAI in MC3T3-E1 cell viability and differentiation. Additionally, the activations of Wnt/β-catenin and MEK/ERK pathways evoked by BAI were both restrained by repression of miR-217. These explorations uncovered that BAI augmented the differentiation of osteoblasts via activations of Wnt/β-catenin and MEK/ERK pathways by ascending miR-217 expression.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Orthopaedics, Heze Municipal Hospital, No. 2888 Caozhou Road, Heze, 274031, China

    Qi Wang, Donglei Shi & Longzhan Xiang

  2. Department of Comprehensive Medical, Heze Infectious Disease Hospital, No. 298 Juyang Road, Heze, 274029, China

    Yuanyuan Geng

  3. Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, No. 109 Xueyuan West Road, Wenzhou, 325000, China

    Qishan Huang

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This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s11010-024-04980-8

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Supplementary Figure 1

BAI expedited rat primary osteoblast viability and osteogenic differentiation. After BAI management (20, 50, and 100 μM), (A) CCK-8 assay for rat primary osteoblast viability evaluation; (B) p-nitrophenyl phosphate (pNPP) assay for ALP activity detection; (C) western blot assay for Runx2 and Ocn protein level estimation; (D and E) RT-qPCR assay for Runx2 and Ocn mRNA expression level determination. *P < 0.05, **P < 0.01, ***P < 0.001 (TIFF 641 kb)

Supplementary Figure 2

BAI promoted osteoblast activity. After BAI management (20, 50, and 100 μM, TRAP activity assay for mature osteoclasts evaluation in (A) MC3T3-E1 cells and (B) rat primary osteoblasts. **P < 0.01, ***P < 0.001 (TIFF 252 kb)

Supplementary Figure 3

BAI expedited the activations of Wnt/β-catenin and MEK/ERK pathways in rat primary osteoblasts. After BAI administration (20, 50, and 100 μM), (A and B) western blot assay for wnt3a and β-catenin protein level measurement; (C and D) western blot assay for p/t-MEK and p/t-ERK protein level determination. *P < 0.05, **P < 0.01 (TIFF 1437 kb)

Supplementary Figure 4

Enhancement of miR-217 was triggered by BAI in rat primary osteoblasts. After BAI management (20, 50, and 100 μM), RT-qPCR assay for the expression level of miR-217 evaluation in rat primary osteoblasts. *P < 0.05, **P < 0.01 (TIFF 171 kb)

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Wang, Q., Shi, D., Geng, Y. et al. RETRACTED ARTICLE: Baicalin augments the differentiation of osteoblasts via enhancement of microRNA-217. Mol Cell Biochem 463, 91–100 (2020). https://doi.org/10.1007/s11010-019-03632-6

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