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Ac154 carried out anti-apoptotic role during AcMNPV infection process in the host insect cells

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Abstract

AcMNPV is the first baculovirus to be sequenced and is considered a model of baculovirus. ac154 is a later expression gene in AcMNPV genome and its function is unknown. In this study, we explored the function of Ac154 in AcMNPV infection process in host Sf9 cells. The results showed that Ac154 was distributed in both nucleus and cytoplasm. Knockout of ac154 did not affect the production of BV, but the yield of progeny virus was reduced, indicating the auxiliary function of Ac154 in virus production. MTT assay showed that Ac154 promoted the proliferation and inhibited apoptosis of Sf9 cells. Overexpression of ac154 gene significantly increased the transcription level of anti-apoptotic gene p35, and delayed the expression of the pro-apoptotic protein SfP53 and reduced its expression level, which indicated its anti-apoptotic role in the host cells. In conclusion, our results demonstrated Ac154 could delay apoptosis process in host cells by regulating the transcription of p35 gene and the expression of SfP53 protein, which provided a more favorable environment for progeny virus replication and packaging, thereby promoting the proliferation of progeny virus. So we provided a potentially improved bac-to-bac eukaryotic protein expression system and biopesticide in this work.

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Acknowledgements

The present work was supported by grants from National Natural Science Foundation of China (No. 31272100) and Natural Science Foundation of Shanxi Province (No. 201801D121193) to YJ Fu. It was also supported by grants from Shanxi ‘1331 project’ Collaborative Innovation Center (1331 CIC) to Y. Fu.

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  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Houlin Bai, Aihua Liang & Yuejun Fu

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  1. Houlin Bai
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  2. Aihua Liang
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  3. Yuejun Fu
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Correspondence to Yuejun Fu.

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Bai, H., Liang, A. & Fu, Y. Ac154 carried out anti-apoptotic role during AcMNPV infection process in the host insect cells. Mol Cell Biochem 463, 79–90 (2020). https://doi.org/10.1007/s11010-019-03631-7

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