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Exosomes derived from microRNA-101-3p-overexpressing human bone marrow mesenchymal stem cells suppress oral cancer cell proliferation, invasion, and migration

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Abstract

Dysregulation of microRNAs (miRNAs) has been found to disrupt the progression of oral cancer. However, which miRNAs are most effective against oral cancer and how these miRNAs should be delivered are major unanswered problems. We aimed at investigating if human bone marrow mesenchymal stem cells (hBMSCs)-derived exosomes affect oral cancer development, and the potential regulatory mechanism associated with COL10A1 and miR-101-3p. COL10A1 was upregulated, while miR-101-3p was downregulated in oral cancer, and miR-101-3p targeted COL10A1 as verified by dual-luciferase reporter gene assay. Meanwhile, exosomes derived from hBMSCs were isolated and then co-cultured with oral cancer cells to identify the role of exosomes, and the results suggested that hBMSCs-derived exosomes overexpressing miR-101-3p inhibited oral cancer progression. Furthermore, tumorigenicity assay in nude mice further confirmed the inhibitory effects of hBMSCs-derived exosomes, loaded with miR-101-3p, on oral cancer, which provides a new theoretical basis in the treatment of oral cancer.

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Acknowledgements

We would also like to thank all participants enrolled in the present study.

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Authors and Affiliations

  1. Department of Stomatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China

    Chun Xie, Lu-Yang Du, Fengyuan Guo & Xiaoshuang Li

  2. Departmant of Stomatology, Zhongnan Hospital of Wuhan University, No. 169, Donghu Road, Wuchang District, Wuhan, 430071, Hubei Province, People’s Republic of China

    Bo Cheng

Authors
  1. Chun Xie
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  2. Lu-Yang Du
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  3. Fengyuan Guo
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  4. Xiaoshuang Li
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  5. Bo Cheng
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Contributions

Chun Xie, Lu-Yang Du and Fengyuan Guo designed the study. Xiaoshuang Li collated the data, designed and developed the database, carried out data analyses and produced the initial draft of the manuscript. Bo Cheng contributed to drafting the manuscript. All authors have read and approved the final submitted manuscript.

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Correspondence to Bo Cheng.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures of animal experiments were also approved by the Ethics Committee of Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, and were in line with the Guide for Care and Use of Laboratory Animals.

Informed consent

This study was approved by the Ethics Committee of Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, and all patients provided signed informed consents.

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Xie, C., Du, LY., Guo, F. et al. Exosomes derived from microRNA-101-3p-overexpressing human bone marrow mesenchymal stem cells suppress oral cancer cell proliferation, invasion, and migration. Mol Cell Biochem 458, 11–26 (2019). https://doi.org/10.1007/s11010-019-03526-7

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  • DOI: https://doi.org/10.1007/s11010-019-03526-7

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