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Protective effect of omeprazole and lansoprazole on β-receptor stimulated myocardial infarction in Wistar rats

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Abstract

We investigated the effect of omeprazole (OPZ) and lansoprazole (LPZ) on the pathophysiology of myocardial necrosis in rats by inspecting a series of indicators like hemodynamic parameters, biochemical estimations and histopathological changes in the myocardial tissue. Rats received either OPZ, LPZ (50 mg/kg/day, p.o.) individually for 7 days with concurrent administration of isoproterenol (ISO) (150 mg/kg, s.c.) on 6th and 7th day of study period to induce myocardial infarction. On the 8th day after measuring hemodynamic parameters, rats were killed and parameters were evaluated. ECG waves were found to be normal in the treatment group. ISO control rats revealed escalation in the oxidative stress as evidenced by depletion in the content of SOD, GSH, catalase and increase in the level of MDA and NO as compared with the normal rats. Treatment with OPZ and LPZ significantly reduced the ROS, indicated by an increase in the endogenous antioxidants and a decrease in NO and MDA levels. ISO control rats showed a significant elevation in the levels of pro-inflammatory cytokine TNF-α as compared to the normal and treatment group of rats. Administration of OPZ and LPZ does not exhibit any significant toxicity. Our findings reveal that multiple doses of OPZ and LPZ may have distinctly minimized the ISO-induced myocardial necrosis by declining the hmodynamic parameters, oxidative stress and pro-inflammatory cytokine TNF-α in myocardial infarcted rats.

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Acknowledgements

The authors also acknowledge the financial support received under Early Career Research Award Scheme (File No. ECR/2016/001243) of Science and Engineering Research Board (SERB), Department of Science and Technology, New Delhi, India.

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Authors and Affiliations

  1. Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Dhule, Shirpur, Maharashtra, 425405, India

    Ashwini S. Patil, Alok D. Singh, Umesh B. Mahajan, Chandragouda R. Patil & Sameer N. Goyal

  2. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, Abu Dhabi, UAE

    Shreesh Ojha

  3. Shri Vile Parle Kelavani Mandal’s Institute of Pharmacy, Dhule, Maharashtra, 424001, India

    Sameer N. Goyal

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  1. Ashwini S. Patil
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  2. Alok D. Singh
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  3. Umesh B. Mahajan
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  4. Chandragouda R. Patil
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  5. Shreesh Ojha
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  6. Sameer N. Goyal
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Correspondence to Sameer N. Goyal.

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The authors declare that there are no conflicts of interest.

Ethical approval

The study protocol used in the study was approved by the Institutional Animal Ethics Committee (Approval No. IAEC/RCPIPER/2017-18/13) of R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, India. The animals were preserved, and inventive procedures were carried out in accordance with the guideline laid down by Committee for the Purpose of Control and Supervision of the Experiments on Animals (CPCSEA) composed under the Prevention of the Cruelty to Animals Act, 1960, Ministry of Environment and Forests, Government of India (Reg. No. IAEC/RCPIPER/2017-18/13).

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Patil, A.S., Singh, A.D., Mahajan, U.B. et al. Protective effect of omeprazole and lansoprazole on β-receptor stimulated myocardial infarction in Wistar rats. Mol Cell Biochem 456, 105–113 (2019). https://doi.org/10.1007/s11010-019-03494-y

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  • DOI: https://doi.org/10.1007/s11010-019-03494-y

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