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The impact of aerobic and anaerobic training regimes on blood pressure in normotensive and hypertensive rats: focus on redox changes

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This study was aimed to assess the impact of aerobic and anaerobic type of exercise on blood pressure and redox status in normotensive and hypertensive rats. After 1 week of preconditioning feeding and 1 week of preconditioning running regimen, Wistar albino rats (n = 72; bw: 270 ± 50 g) were randomly assigned to three groups according to running protocol (high-intensity interval training (HIIT) or moderate-intensity training (MIT)): sedentary control, MIT, HIIT; spontaneous hypertensive sedentary control (SHR), SHR + MIT and SHR + HIIT. Blood pressure (BP) measurement was performed by a tail-cuff noninvasive method BP system. After 48 h of rest following the final training, the rats were fasted for 24 h and sacrificed under ketamine/xylazine anesthesia and blood samples were collected. The level of the next prooxidants were measured: superoxide anion radical (O2−); hydrogen peroxide (H2O2); nitrite level (NO2−) and index of lipid peroxidation (thiobarbituric acid reactive substances), and the activity of antioxidative enzymes: reduced glutathione (GSH) superoxide dismutase (SOD) and catalase (CAT) activity. After the last week of running, HIIT strongly affected SP, DP, and HR in SHR rats compared to other hypertensive rats, as well as after MIT in normotensive conditions. We have found that HIIT training protocol induced a higher increase of O2− and H2O2 as compared to MIT. Findings of the present study pointed out that contrary to normotensive conditions, in hypertensive conditions both training regimes reduced the BP levels, which was more prominent in case of HIIT. In addition, MIT seems to be connected with milder disturbance of pro-oxidant production and better antioxidant response.

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Acknowledgements

This work was supported by Faculty of Medical Sciences, University of Kragujevac, Serbia (JP 01/15).

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Authors and Affiliations

  1. Medical College of Applied Sciences in Zemun, Belgrade, Serbia

    Biljana Jakovljevic

  2. Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

    Tamara Nikolic Turnic, Nevena Jeremic, Jovana Jeremic, Jovana Bradic & Marko Ravic

  3. Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica Str. 69, P.O. Box 124, 34000, Kragujevac, Serbia

    Vladimir Lj. Jakovljevic & Vladimir Zivkovic

  4. Department of Human Pathology, 1st Moscow State Medical, University IM Sechenov, Trubetskaya Street 8, Str. 2, Moscow, 11999, Russian Federation

    Vladimir Lj. Jakovljevic

  5. Faculty of Stomatology in Pancevo, University Business Academy Novi Sad, Novi Sad, Serbia

    Djordje Jelic

  6. Department of Physiology, Faculty of Sport and Physical Education, University of Nis, Carnojeviceva 10A, Nis, 18000, Serbia

    Dragan Radovanovic

  7. Instutute of Normal and Pathological Physiology, Slovac Academy of Sciences, Bratislava, Slovakia

    Olga Pechanova

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  1. Biljana Jakovljevic
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Correspondence to Vladimir Lj. Jakovljevic.

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Jakovljevic, B., Nikolic Turnic, T., Jeremic, N. et al. The impact of aerobic and anaerobic training regimes on blood pressure in normotensive and hypertensive rats: focus on redox changes. Mol Cell Biochem 454, 111–121 (2019). https://doi.org/10.1007/s11010-018-3457-y

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