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miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR

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Abstract

MicroRNAs are members of the family of non-coding small RNAs that regulate gene expression either by inhibiting mRNA translation or by promoting mRNA degradation at the post-transcriptional level. They play an important role in the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into adipocytes. However, the role of microRNAs in this process remains to be poorly understood. Here, we observed that miR-377-3p expression was markedly decreased during adipogenic differentiation of hMSCs. Overexpression of miR-377-3p decreased adipocyte differentiation and downregulated the expression of adipogenic markers. Meanwhile, bioinformatics-based studies suggested that LIFR is a target of miR-377-3p. Further analysis confirmed that expression of LIFR present markedly increased during adipogenic differentiation of hMSCs. In addition, downregulation expression of LIFR significantly inhibited the process of adipocyte differentiation. To confirm the relation between miR-377-3p and LIFR, luciferase reporter assays were carried out. The results indicated that miR-377-3p bound directly to the 3′-untranslated region of LIFR. These data indicate that miR-377-3p suppressed adipogenesis of hMSCs by targeting LIFR, which provides novel insights into the molecular mechanism of miRNA-mediated cellular differentiation.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grants No. 81460221) and Jiangxi Province Natural Science Foundation of China (No. 20161BAB205197).

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Authors and Affiliations

  1. Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiujiang, Jiangxi, People’s Republic of China

    Xingnuan Li, Yaofang Yang, Xiaoyuan Xu, Jun Mei, Jianyun Liu, Xinping Wang, Jie Zhang, Ping Wu, Weidong Li, Jianjun Xiong & Tao Wang

  2. Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang, Jiangxi, People’s Republic of China

    Ruiqiao Yan

  3. School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, People’s Republic of China

    Liyun Gao

  4. Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, People’s Republic of China

    Zhijun Zhao

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  1. Xingnuan Li
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Correspondence to Jianjun Xiong or Tao Wang.

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Li, X., Yang, Y., Yan, R. et al. miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR. Mol Cell Biochem 449, 295–303 (2018). https://doi.org/10.1007/s11010-018-3366-0

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  • DOI: https://doi.org/10.1007/s11010-018-3366-0

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