Abstract
Upregulation of A-kinase-interacting protein 1 (AKIP1) has been observed in breast and esophageal cancers, indicating that AKIP1 may be a potent oncogenic protein. However, the role of AKIP1 in cervical cancer still remains unknown. This study aimed to explore the role of AKIP1 in cervical cancer and to investigate the underlying mechanism of AKIP1 in tumor growth. Expression of AKIP1 in cervical cancer cells was determined by qRT-PCR and western blotting. Cell-Light EdU and colony formation assays were used to determine cell proliferation. CXCL1 and CXCL8 proteins were quantified by ELISA kits. Western blotting and qRT-PCR were used to examine the alterations in signaling-related proteins and mRNA, respectively. Endothelial cell tube formation assay was performed to evaluate the effect of AKIP1 on angiogenesis. A BALB/c nude mouse xenograft model was used to evaluate the role of AKIP1 in vivo. Cancer cell proliferation was inhibited and tumor growth and angiogenesis restrained in BALB/c nude mice by suppressing AKIP1 expression in cervical cancer cell lines. In addition, overexpression of AKIP1 in cervical cancer cells elevated the levels of CXCL1, CXCL2, and CXCL8. These three chemokines were not only involved in endothelial tube formation by binding to the endothelial receptor CXCR2, but also in cervical cancer cell proliferation and clone formation, which were induced by overexpression of AKIP1. Furthermore, we found that AKIP1-induced chemokine expression was decreased by an inhibitor of nuclear factor kappa-B kinase subunit β. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-κB-dependent chemokines CXCL1, CXCL2, and CXCL8.
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Siegel R, Naishadham D, Jemal A (2012) Cancer statistics, 2012. CA Cancer J Clin 62(1):10–29. https://doi.org/10.3322/caac.20138
Bosch FX, de Sanjose S (2007) The epidemiology of human papillomavirus infection and cervical cancer. Dis Mark 23(4):213–227
Barakat RR, Markman M, Randall M (2009) Principles and practice of gynecologic oncology. Lippincott Williams & Wilkins, Philadelphia
Bremnes RM, Camps C, Sirera R (2006) Angiogenesis in non-small cell lung cancer: the prognostic impact of neoangiogenesis and the cytokines VEGF and bFGF in tumours and blood. Lung Cancer 51(2):143–158. https://doi.org/10.1016/j.lungcan.2005.09.005
Scotton CJ, Wilson JL, Milliken D, Stamp G, Balkwill FR (2001) Epithelial cancer cell migration: a role for chemokine receptors? Cancer Res 61(13):4961–4965
Kitching R, Li H, Wong MJ, Kanaganayakam S, Kahn H, Seth A (2003) Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein. Biochim Biophys Acta 1625(1):116–121
Sastri M, Barraclough DM, Carmichael PT, Taylor SS (2005) A-kinase-interacting protein localizes protein kinase A in the nucleus. Proc Natl Acad Sci USA 102(2):349–354. https://doi.org/10.1073/pnas.0408608102
Sastri M, Haushalter KJ, Panneerselvam M, Chang P, Fridolfsson H, Finley JC, Ng D, Schilling JM, Miyanohara A, Day ME, Hakozaki H, Petrosyan S, Koller A, King CC, Darshi M, Blumenthal DK, Ali SS, Roth DM, Patel HH, Taylor SS (2013) A kinase interacting protein (AKIP1) is a key regulator of cardiac stress. Proc Natl Acad Sci USA 110(5):E387–E396. https://doi.org/10.1073/pnas.1221670110
Benedetti Panici P, Basile S, Angioli R (2009) Pelvic and aortic lymphadenectomy in cervical cancer: the standardization of surgical procedure and its clinical impact. Gynecol Oncol 113(2):284–290. https://doi.org/10.1016/j.ygyno.2008.12.014
Gao N, Hibi Y, Cueno M, Asamitsu K, Okamoto T (2010) A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling. J Biol Chem 285(36):28097–28104. https://doi.org/10.1074/jbc.M110.116566
Schmitt J, Matei D (2012) Targeting angiogenesis in ovarian cancer. Cancer Treat Rev 38(4):272–283. https://doi.org/10.1016/j.ctrv.2011.06.004
Acharyya S, Oskarsson T, Vanharanta S, Malladi S, Kim J, Morris PG, Manova-Todorova K, Leversha M, Hogg N, Seshan VE, Norton L, Brogi E, Massague J (2012) A CXCL1 paracrine network links cancer chemoresistance and metastasis. Cell 150(1):165–178. https://doi.org/10.1016/j.cell.2012.04.042
Bolitho C, Hahn MA, Baxter RC, Marsh DJ (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer 17(4):929–940. https://doi.org/10.1677/ERC-10-0107
Merritt WM, Lin YG, Spannuth WA, Fletcher MS, Kamat AA, Han LY, Landen CN, Jennings N, De Geest K, Langley RR, Villares G, Sanguino A, Lutgendorf SK, Lopez-Berestein G, Bar-Eli MM, Sood AK (2008) Effect of interleukin-8 gene silencing with liposome-encapsulated small interfering RNA on ovarian cancer cell growth. J Natl Cancer Inst 100(5):359–372. https://doi.org/10.1093/jnci/djn024
Yang G, Rosen DG, Zhang Z, Bast RC Jr, Mills GB, Colacino JA, Mercado-Uribe I, Liu J (2006) The chemokine growth-regulated oncogene 1 (Gro-1) links RAS signaling to the senescence of stromal fibroblasts and ovarian tumorigenesis. Proc Natl Acad Sci USA 103(44):16472–16477. https://doi.org/10.1073/pnas.0605752103
Willmott LJ, Monk BJ (2009) Cervical cancer therapy: current, future and anti-angiogensis targeted treatment. Expert Rev Anticancer Ther 9(7):895–903. https://doi.org/10.1586/era.09.58
Mo D, Li X, Li C, Liang J, Zeng T, Su N, Jiang Q, Huang J (2016) Overexpression of AKIP1 predicts poor prognosis of patients with breast carcinoma and promotes cancer metastasis through Akt/GSK-3beta/Snail pathway. Am J Transl Res 8(11):4951–4959
Yu H, Tigchelaar W, Koonen DP, Patel HH, de Boer RA, van Gilst WH, Westenbrink BD, Sillje HH (2013) AKIP1 expression modulates mitochondrial function in rat neonatal cardiomyocytes. PLoS ONE 8(11):e80815. https://doi.org/10.1371/journal.pone.0080815
Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J (2000) Vascular-specific growth factors and blood vessel formation. Nature 407(6801):242–248. https://doi.org/10.1038/35025215
Bergers G, Benjamin LE (2003) Tumorigenesis and the angiogenic switch. Nat Rev Cancer 3(6):401–410. https://doi.org/10.1038/nrc1093
Randall LM, Monk BJ, Darcy KM, Tian C, Burger RA, Liao SY, Peters WA, Stock RJ, Fruehauf JP (2009) Markers of angiogenesis in high-risk, early-stage cervical cancer: a gynecologic oncology group study. Gynecol Oncol 112(3):583–589. https://doi.org/10.1016/j.ygyno.2008.11.013
Kodama J, Seki N, Tokumo K, Hongo A, Miyagi Y, Yoshinouchi M, Okuda H, Kudo T (1999) Vascular endothelial growth factor is implicated in early invasion in cervical cancer. Eur J Cancer 35(3):485–489
Pietras K, Pahler J, Bergers G, Hanahan D (2008) Functions of paracrine PDGF signaling in the proangiogenic tumor stroma revealed by pharmacological targeting. PLoS Med 5(1):e19. https://doi.org/10.1371/journal.pmed.0050019
Murdoch C, Muthana M, Coffelt SB, Lewis CE (2008) The role of myeloid cells in the promotion of tumour angiogenesis. Nat Rev Cancer 8(8):618–631. https://doi.org/10.1038/nrc2444
Flanagan K, Kaufman HL (2002) Chemokines and cancer. Cancer Invest 20(5–6):825–834
Kodama J, Kusumoto T, Seki N, Matsuo T, Ojima Y, Nakamura K, Hongo A, Hiramatsu Y (2007) Association of CXCR4 and CCR7 chemokine receptor expression and lymph node metastasis in human cervical cancer. Ann Oncol 18(1):70–76. https://doi.org/10.1093/annonc/mdl342
Balkwill F (2004) Cancer and the chemokine network. Nat Rev Cancer 4(7):540–550. https://doi.org/10.1038/nrc1388
Wang Y, Xu RC, Zhang XL, Niu XL, Qu Y, Li LZ, Meng XY (2012) Interleukin-8 secretion by ovarian cancer cells increases anchorage-independent growth, proliferation, angiogenic potential, adhesion and invasion. Cytokine 59(1):145–155. https://doi.org/10.1016/j.cyto.2012.04.013
Hayden MS, Ghosh S (2008) Shared principles in NF-kappaB signaling. Cell 132(3):344–362. https://doi.org/10.1016/j.cell.2008.01.020
Duckworth C, Zhang L, Carroll SL, Ethier SP, Cheung HW (2016) Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression. Oncogene 35(31):4036–4047. https://doi.org/10.1038/onc.2015.472
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This study was supported by a Fund Project of the Changning District Science and Technology Commission (CNKW2015Y16).
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Zhang, W., Wu, Q., Wang, C. et al. AKIP1 promotes angiogenesis and tumor growth by upregulating CXC-chemokines in cervical cancer cells. Mol Cell Biochem 448, 311–320 (2018). https://doi.org/10.1007/s11010-018-3335-7
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DOI: https://doi.org/10.1007/s11010-018-3335-7