Chemopreventive potential of esculetin in 7,12-dimethylbenz(a)anthracene-induced hamster buccal pouch carcinogenesis
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7,12-Dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch carcinogenesis is widely preferred to assess the tumor-inhibiting efficacy of the medicinal plants or their constituents. The present study explores the tumor-inhibiting potential of esculetin by utilizing the status of lipid peroxidation by products (thiobarbituric acid reactive substances), antioxidants (vitamin E, reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase), and phase I and phase II detoxification agents as biochemical end points and by using histopathological studies as well in DMBA-induced hamster buccal pouch carcinogenesis. Oral tumors developed in the buccal pouch were subjected to histopathological studies, and were confirmed as oral squamous cell carcinoma. Hamsters treated with DMBA alone showed an abnormal pattern of lipid peroxidation, antioxidants, and detoxification agents as compared to control hamsters. The status of the above-mentioned biochemical markers and histopathological abnormalities were found to be reversed in DMBA + esculetin-treated hamsters. The result of the present study thus indicates the tumor preventive potential of esculetin in DMBA-induced oral carcinogenesis.
KeywordsOral cancer Esculetin Lipid peroxidation Antioxidants
Financial assistance from Indian Council of Medical Research, New Delhi is gratefully acknowledged. The authors gratefully acknowledge UGC-SAP and DST-FIST support in the form of providing necessary infrastructural facilities to carry out the work successfully.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- 3.Manoharan S, Sindhu G, Nirmal MR, Vetrichelvi V, Balakrishnan S (2011) Protective effect of berberine on expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers during 7,12 dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis. Pak J Biol Sci 14(20):918–932CrossRefPubMedGoogle Scholar
- 12.Arora R, Sawney S, Saini V, Steffi C, Tiwari M, Saluja D (2016) Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1. Mol Cancer 15:(64):1–15Google Scholar
- 28.Ernster L (1967) DT-Diaphorase. In: Estabrook RW, Pullman ME (ed) Methods in enzymology, vol 10. Academic Press, New York, pp 309–317Google Scholar
- 30.Waris G, Ahsan H (2006) Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog 5(14):1–8Google Scholar
- 35.Manoharan S, Balakrishnan S, Menon VP, Alias LM, Reena AR (2009) Chemopreventive efficacy of curcumin and piperine during 7,12 dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Singap Med J 50(2):139–146Google Scholar