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Novel miR-sc4 regulates the proliferation and migration of Schwann cells by targeting Cdk5r1

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Abstract

The proliferation and migration of Schwann cells are critical for the repair and regeneration of injured peripheral nerves. Noncoding RNAs, especially microRNAs (miRNAs), have been demonstrated to participate in regulating the biological behaviors of Schwann cells. Numerous differentially expressed novel miRNAs have been identified in the injured sciatic nerve stumps previously by Solexa sequencing. In the current research, we studied the biological function of a novel miRNA, miR-sc4, in detail. Outcomes from proliferation and migration assays suggested that miR-sc4 played an inhibitory role on the proliferation and migration of Schwann cells. Results from bioinformatic analysis, luciferase reporter assay, and rescue experiments suggested that miR-sc4 executed its effect through directly targeting cyclin-dependent kinase 5 activator 1 (Cdk5r1). Collectively, our current study revealed the biological functions of a novel miRNA, showed the effect of miR-sc4 in Schwann cell phenotypic changes, and thus indicated the involvement of miRNAs in peripheral nerve repair and regeneration.

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Funding

This study was supported by the Natural Science Foundation of Jiangsu Province, China (BK20150409); Pre-research Project Funding of Nantong University (16ZY13); Scientific Research Project Funding of Nantong University (Project Code: 13260128); Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX17-1910); and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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    Authors and Affiliations

    1. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, China

      Tianmei Qian, Xinghui Wang, Yaxian Wang, Pan Wang, Qianyan Liu, Jie Liu & Sheng Yi

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    1. Tianmei Qian
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    2. Xinghui Wang
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    3. Yaxian Wang
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    4. Pan Wang
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    7. Sheng Yi
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    Contributions

    Conceived and designed the experiments: TQ and SY: Performed the experiments: TQ, XW, YW, PW, QL, and SY: Analyzed the data: TQ, XW, and SY: Contributed to applying reagents/materials/analysis tools: SY; Wrote the manuscript: TQ, XW, JL, and SY.

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    Correspondence to Sheng Yi.

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    The authors declare that they have no conflict of interest.

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    Tianmei Qian and Xinghui Wang have equal contribution to this work.

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    Qian, T., Wang, X., Wang, Y. et al. Novel miR-sc4 regulates the proliferation and migration of Schwann cells by targeting Cdk5r1. Mol Cell Biochem 447, 209–215 (2018). https://doi.org/10.1007/s11010-018-3305-0

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