Molecular and Cellular Biochemistry

, Volume 442, Issue 1–2, pp 11–18 | Cite as

Necroptosis may be a novel mechanism for cardiomyocyte death in acute myocarditis

  • Fei Zhou
  • Xuejun Jiang
  • Lin Teng
  • Jun Yang
  • Jiawang Ding
  • Chao He
Article

Abstract

In this study, we investigated the roles of RIP1/RIP3 mediated cardiomyocyte necroptosis in CVB3-induced acute myocarditis. Serum concentrations of creatinine kinase (CK), CK-MB, and cardiac troponin I were detected using a Hitachi Automatic Biochemical Analyzer in a mouse model of acute VMC. Histological changes in cardiac tissue were observed by light microscope and expression levels of RIP1/RIP3 in the cardiac tissue were detected via Western blot and immunohistochemistry. The data showed that RIP1/RIP3 was highly expressed in cardiomyocytes in the acute VMC mouse model and that the necroptosis pathway specific blocker, Nec-1, dramatically reduced the myocardial damage by downregulating the expression of RIP1/RIP3. These findings provide evidence that necroptosis plays a significant role in cardiomyocyte death and it is a major pathway for cell death in acute VMC. Blocking the necroptosis pathway may serve as a new therapeutic option for the treatment of acute viral myocarditis.

Keywords

Viral myocarditis Coxsackievirus B3 Necroptosis receptor-interacting proteins 

Notes

Acknowledgements

The authors would like to thank Cunyu Cao and Hui Zhao for their technical support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Not applicable.

Research involving animals

All procedures, experiments and animal care were approved by the Institutional Animal Care and Use Committees of Wuhan University and conformed to the Guide for the Care and Use of Laboratory Animals by the National Institutes of Health.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Fei Zhou
    • 1
  • Xuejun Jiang
    • 1
  • Lin Teng
    • 2
  • Jun Yang
    • 2
  • Jiawang Ding
    • 2
  • Chao He
    • 2
  1. 1.Department of CardiologyRenmin Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of Cardiology, The First College of Clinical Medical SciencesChina Three Gorges UniversityYichangChina

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