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Enhanced migration of murine fibroblast-like 3T3-L1 preadipocytes on type I collagen-coated dish is reversed by silibinin treatment

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Abstract

Migration of fibroblast-like preadipocytes is important for the development of adipose tissue, whereas excessive migration is often responsible for impaired adipose tissue related with obesity and fibrotic diseases. Type I collagen (collagen I) is the most abundant component of extracellular matrix and has been shown to regulate fibroblast migration in vitro, but its role in adipose tissue is not known. Silibinin is a bioactive natural flavonoid with antioxidant and antimetastasis activities. In this study, we found that type I collagen coating promoted the proliferation and migration of murine 3T3-L1 preadipocytes in a dose-dependent manner, implying that collagen I could be an extracellular signal. Regarding the mechanisms of collagen I-stimulated 3T3-L1 migration, we found that NF-κB p65 is activated, including the increased nuclear translocation of NF-κB p65 as well as the upregulation of NF-κB p65 phosphorylation and acetylation, accompanied by the increased expressions of proinflammatory factors and the generation of reactive oxygen species (ROS). Reduction of collagen I-enhanced migration of cells by treatment with silibinin was associated with suppression of NF-κB p65 activity and ROS generation, and negatively correlated with the increasing sirt1 expression. Taken together, the enhanced migration of 3T3-L1 cells induced on collagen I-coated dish is mediated by the activation of NF-κB p65 function and ROS generation that can be alleviated with silibinin by upregulation of sirt1, leading to the repression of NF-κB p65 function and ROS generation.

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Authors and Affiliations

  1. China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, Liaoning, China

    Xiaoling Liu, Qian Xu, Weiwei Liu, Guodong Yao, Yeli Zhao, Fanxing Xu, Toshihiko Hayashi & Takashi Ikejima

  2. Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang, China

    Guodong Yao

  3. Nippi Research Institute of Biomatrix, Nippi, Incorporated, Toride, Japan

    Hitomi Fujisaki & Shunji Hattori

  4. Department of Medical Education and Primary Care, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Shin-ichi Tashiro

  5. Department of Clinical and Pharmaceutical Sciences, Showa Pharmaceutical University, Tokyo, Japan

    Satoshi Onodera

  6. Waseda University Joint Institution for Advanced Biomedical Sciences, Tokyo Women’s Medical University, Tokyo, Japan

    Masayuki Yamato

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  1. Xiaoling Liu
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Liu, X., Xu, Q., Liu, W. et al. Enhanced migration of murine fibroblast-like 3T3-L1 preadipocytes on type I collagen-coated dish is reversed by silibinin treatment. Mol Cell Biochem 441, 35–62 (2018). https://doi.org/10.1007/s11010-017-3173-z

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