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Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPKα-FOXO1 signalling pathway in bovine intramuscular adipocytes

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Abstract

Sirtuin type 1 (SIRTl) and AMP-activated protein kinase (AMPK) play important roles in regulating energy metabolism, cell proliferation and differentiation, ageing, apoptosis, and metabolism. The effect of 100, 200, and 400 μm Resveratrol (RES), an activator of SIRT1, on apoptosis of bovine intramuscular adipocytes was investigated by nuclear staining, flow cytometry, quantitative real-time polymerase chain reaction, and western blotting. Results show that RES inhibited adipogenesis, decreased cell viability, and increased apoptotic rates in a dose-dependent way. RES up-regulated SIRT1, AMPKα, forkhead box O1 (FOXO1), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), caspase-3, and Bax; and down-regulated peroxisome proliferator-activated receptor-gamma (PPARγ), fatty acid synthase (FAS), and Bcl-2, at both mRNA and protein level. The effect of RES was abolished by addition of sirtinol (an inhibitor of SIRT1). This is the first study demonstrating a role for AMPK-SIRT1-FOXO1 signalling pathway in regulating apoptosis in bovine intramuscular adipocytes. Our findings provide important information on the mechanism by which RES controls deposition of cattle intramuscular fat via adipocyte apoptosis.

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (31402098, 31601966), the Sustentative Research Project of China’s Ministry of Science and Technology (2015BAD03B04), and the Agricultural Science and Technology Innovation Project of Shandong’s Academy of Agricultural Sciences (CXGC2016A04). We would like to thank Editage (www.editage.cn) for English language editing.

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  1. Xiaomu Liu and Hongbo Zhao are contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Jinan, 250100, Shandong Province, China

    Xiaomu Liu, Hongbo Zhao, Qing Jin, Wei You, Haijian Cheng, Yifan Liu, Enliang Song, Guifen Liu, Xiuwen Tan, Xianglun Zhang & Fachun Wan

  2. Shandong Key Lab of Animal Disease Control and Breeding, Sangyuan Road Number 8, Jinan, 250100, Shandong Province, China

    Xiaomu Liu, Hongbo Zhao, Qing Jin, Wei You, Haijian Cheng, Yifan Liu, Enliang Song, Guifen Liu, Xiuwen Tan, Xianglun Zhang & Fachun Wan

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  1. Xiaomu Liu
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Correspondence to Fachun Wan.

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Liu, X., Zhao, H., Jin, Q. et al. Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPKα-FOXO1 signalling pathway in bovine intramuscular adipocytes. Mol Cell Biochem 439, 213–223 (2018). https://doi.org/10.1007/s11010-017-3149-z

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