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Pre- and post-prandial expression of genes involved in lipid metabolism at the end of the overfeeding period of mule ducks

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Abstract

In palmipeds, overfeeding leads to hepatic steatosis, also called “foie gras” which is the result of many metabolic mechanisms. In order to understand these mechanisms, we decided to measure the expression of genes implicated in lipid metabolism during 12 hours (h) following the last meal of the overfeeding period. We have shown that there is a precocious expression (within 2 h) of fatty acid synthase and acyl CoA synthetase long-chain 1 in liver and muscle of mule ducks in addition with a later peak. Furthermore, di-acyl glycerol acyl transferase presents the highest induction of expression in liver and it is overexpressed quite a long time, positioning this enzyme as a key factor in hepatic steatosis. These observations are quite similar in muscle. Lipoprotein secretion is upregulated later in postprandial period, with an upregulation of apolipoprotein and microsomal triglycerides transfer protein beginning at 5 h in liver or muscle. Regarding hepatic re-uptake of lipid, lesser variations are observed, suggesting that fatty acid binding protein and very low-density lipoprotein receptor (VLDLR) are already at their maximum expression specifically in these tissues. In muscle, VLDLR and LDLR upregulation is observed 5 h after the meal, associated with an overexpression in the adipose tissue of lipase maturation factor 1 involved in the maturation of lipoprotein lipase. These findings will allow us to better understand the kinetic treatment in lipid metabolism after a meal in overfed ducks. This first report on kinetic expression will allow researcher to better target their sampling time knowing the optimal point of expression of each gene.

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Acknowledgements

We thank the “Conseil Général des Landes” and the “Comité Interprofessionnel des Palmipèdes à Foie Gras” (CIFOG) for financing this work. We also thank the technical staff of INRA Artiguères for rearing ducks (Certificate of Authorization to Experiment on Living animals, No. B40-037-1, Ministry of Agriculture and Fish Products, ethic committee Aquitaine birds and fish No. C2EA-73). We are grateful to Frédéric Martins and Jean-José Maoret for performing Fluidigm analysis (Génopole Toulouse/Midi-pyrénées, Plateau Transcriptomique Quantitative (TQ), Toulouse, France). We finally thank Patrick Daniel, Karine Bellet, and Martine Chague of the laboratory Pyrénées Landes (LPL, Mont de Marsan, France) to let us use their material.

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Authors and Affiliations

  1. UMR 1419 INRA UPPA NuMéA, 371 Rue du Ruisseau, 40000, Mont de Marsan, France

    Tavernier Annabelle, Ricaud Karine, Gontier Karine & Davail Stéphane

  2. UMR 1419 INRA UPPA NuMéA, Quartier Ibarron, 64310, Saint Pée sur Nivelle, France

    Tavernier Annabelle, Ricaud Karine, Gontier Karine & Davail Stéphane

  3. INRA UEPFG, 1076 Route de Haut Maucq, 40280, Benquet, France

    Bernadet Marie-Dominique

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  1. Tavernier Annabelle
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Correspondence to Gontier Karine.

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Annabelle, T., Karine, R., Marie-Dominique, B. et al. Pre- and post-prandial expression of genes involved in lipid metabolism at the end of the overfeeding period of mule ducks. Mol Cell Biochem 438, 111–121 (2018). https://doi.org/10.1007/s11010-017-3118-6

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  • DOI: https://doi.org/10.1007/s11010-017-3118-6

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