Molecular and Cellular Biochemistry

, Volume 436, Issue 1–2, pp 49–58 | Cite as

Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet

  • Aleksandra Jovanovic
  • Milan Obradovic
  • Emina Sudar Milovanovic
  • Alan J. Stewart
  • Samantha J. Pitt
  • Dragan Alavantic
  • Ema Aleksic
  • Esma R. Isenovic
Article
  • 143 Downloads

Abstract

The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.

Keywords

High-fat diet Estradiol Na+/K+-ATPase Obesity RhoA/ROCK signalling Female 

Abbreviations

Akt

Protein kinase B

CD36

Cluster of differentiation 36

CVD

Cardiovascular disease

T2DM

Type 2 diabetes mellitus

ERα

Estrogen receptor-α

HF diet

High-fat diet

HOMA-IR

HOMA-index of insulin resistance

HOMA-β

HOMA-index of β-cell function

iNOS

Inducible nitric oxide synthase

INS

Insulin

IR

Insulin resistance

IRS

Insulin receptor substrate

Na+/K+-ATPase

Sodium/potassium–adenosine–triphosphatase

PI3K

Phosphatidylinositol 3-kinase

RhoA

Ras homolog gene family, member A

ROCK

Rho kinase

Notes

Acknowledgements

This work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Laboratory of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences VincaUniversity of BelgradeBelgradeSerbia
  2. 2.School of MedicineUniversity of St AndrewsSt AndrewsUK
  3. 3.Faculty of Dentistry in PancevoUniversity Business AcademyBelgradeSerbia

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