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Molecular and Cellular Biochemistry

, Volume 436, Issue 1–2, pp 39–48 | Cite as

Aggregatibacter actinomycetemcomitans regulates the expression of integrins and reduces cell adhesion via integrin α5 in human gingival epithelial cells

  • Shinsuke Kochi
  • Keisuke Yamashiro
  • Shoichi Hongo
  • Tadashi Yamamoto
  • Yuki Ugawa
  • Masayuki Shimoe
  • Mari Kawamura
  • Chiaki Hirata-Yoshihara
  • Hidetaka Ideguchi
  • Hiroshi Maeda
  • Shogo Takashiba
Article

Abstract

Gingival epithelial cells form a physiological barrier against bacterial invasion. Excessive bacterial invasion destroys the attachment between the tooth surface and the epithelium, resulting in periodontitis. Integrins play a significant role in cell attachment; therefore, we hypothesized that bacterial infection might decrease the expressions of these integrins in gingival epithelial cells, resulting in reduced cell adhesion. Immortalized human gingival epithelial cells were co-cultured with Aggregatibacter actinomycetemcomitans Y4 (Aa Y4), and the gene expression levels of IL-8, proliferating cell nuclear antigen (PCNA), and integrins (α2, α3, α5, β4, and β6) were measured using quantitative reverse transcription polymerase chain reaction. Expression of PCNA and integrins, except integrin α5, was significantly downregulated, while expression of IL-8 and integrin α5 was significantly upregulated in the cells co-cultured with Aa Y4. The number of adherent cells significantly decreased when co-cultured with Aa Y4, as determined using cell adhesion assays. In the cells co-cultured with Aa Y4 and an integrin α5 neutralizing antibody, there was no effect on the expression of IL-8 and PCNA, while the expressions of integrins α2, α3, β4, and β6, and the number of adherent cells did not decrease. The number of invading bacteria in the cells was reduced in the presence of the antibody and increased in the presence of TLR2/4 inhibitor. Therefore, integrin α5 might be involved in Aa Y4 invasion into gingival epithelial cells, and the resulting signal transduction cascade reduces cell adhesion by decreasing the expression of integrins, while the TLR2/4 signaling cascade regulates IL-8 expression.

Keywords

Aggregatibacter actinomycetemcomitans Cell adhesion Integrin Infection model Gingival epithelial cell Periodontal disease 

Notes

Acknowledgements

This study is supported by a Grant-in-Aid for Research Activity (Start-up Number: JP22890119) and a Grant-in-Aid for Young Scientists (B; Number: JP24792327). We would like to thank Editage (www.editage.jp) for English language editing.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no duality of interest associated with this manuscript.

Supplementary material

11010_2017_3076_MOESM1_ESM.tiff (45.8 mb)
Supplementary material 1 (TIFF 46854 kb)
11010_2017_3076_MOESM2_ESM.tiff (45.8 mb)
Supplementary material 2 (TIFF 46854 kb)
11010_2017_3076_MOESM3_ESM.docx (28 kb)
Supplementary material 3 (DOCX 28 kb)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Shinsuke Kochi
    • 1
  • Keisuke Yamashiro
    • 1
  • Shoichi Hongo
    • 1
  • Tadashi Yamamoto
    • 2
  • Yuki Ugawa
    • 1
  • Masayuki Shimoe
    • 1
  • Mari Kawamura
    • 1
  • Chiaki Hirata-Yoshihara
    • 1
  • Hidetaka Ideguchi
    • 1
  • Hiroshi Maeda
    • 3
  • Shogo Takashiba
    • 1
  1. 1.Department of Pathophysiology-Periodontal ScienceOkayama University Graduate School of Medicine, Dentistry, and Pharmaceutical SciencesOkayamaJapan
  2. 2.Department of Periodontics and EndodonticsOkayama University HospitalOkayamaJapan
  3. 3.Deparment of EndodonticsOsaka Dental UniversityOsakaJapan

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