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Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography

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Abstract

Cytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and divided into 2 groups: those with ≥50% occlusion in at least one coronary artery [Angiography (+)] or <50% obstruction in coronary arteries [Angiography (−)]. Also 120 healthy subjects were enrolled as control group. Lipid profile, fasting blood glucose, body mass index, and blood pressure were evaluated in all the subjects. An Evidence Investigator® was used for measuring 12 cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, MCP-1, IFN-γ, EGF, VEGF) using sandwich chemiluminescent assays. Univariate analysis, multivariate regression models, ROC, and Kaplan–Meier survival curves were used for exploring the candidate markers in CAD patients. Serum level of IFN-γ, IL-4, MCP-1, EGF, IL-6, and IL-8 were markedly higher in angiogram-positive patients, while VEGF concentrations were significantly (P < 0.05) lower, compared to control group. ROC analysis for MCP-1 showed that a cut-off of 61.95 pg/mL had 91% sensitivity and 91% specificity for predicting CAD patients. Moreover, >2.16 pg/mL IL-6 had a > 94% sensitivity and 70% specificity in predicting 2 years mortality in the subjects with a serum MCP-1 > 61.95 pg/ mL, and patients having IL-6/MCP-1 combination had a shorter survival.Our findings demonstrate that CAD patients with serum MCP-1 and IL-6 levels of >61.95 and >2.16 pg/mL had a higher mortality with 94.1% sensitivity and 70.5% specificity for predicting mortality in CAD patients.

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Acknowledgements

The Mashhad University of Medical Science Research Council supported this research (Fund Number MUMS/900671). This study is the subject thesis of Mr. Mohammad Tajfard, which is the PhD Candidate of the community health in University Putra Malaysia. Authors would like to thank Center for International Scientific Studies and Collaboration for their support.

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Authors and Affiliations

  1. Department of Health Education and Health Promotion, School of Health, Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Tajfard

  2. Department of Community Health, Faculty of Medicine and Health Sciences, University Putra Malaysia, Seri Kembangan, Malaysia

    Mohammad Tajfard & Latiffah A. Latiff

  3. Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Hamid Reza Rahimi & Maryam Mardan-Nik

  4. Cardiovascular Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohsen Moohebati, Mehdi Hasanzadeh & Ahmad Sarreshtehdar Emrani

  5. Department of Biostatistics & Epidemiology, School of Health, Management & Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Habibollah Esmaeily & Ali Taghipour

  6. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran

    Sayed Reza Mirhafez

  7. Division of Medical Education, Brighton & Sussex Medical School, Falmer, Brighton, Sussex, BN1 9PH, UK

    Gordon A. Ferns

  8. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Elham Mohammadzadeh, Amir Avan & Majid Ghayour-Mobarhan

  9. Department of Biology, Biochemistry Unit, Alzahra University, Tehran, IR, Iran

    Parichehr Hanachi

Authors
  1. Mohammad Tajfard
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  2. Latiffah A. Latiff
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  3. Hamid Reza Rahimi
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  4. Mohsen Moohebati
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  5. Mehdi Hasanzadeh
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  6. Ahmad Sarreshtehdar Emrani
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  7. Habibollah Esmaeily
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  8. Ali Taghipour
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  9. Sayed Reza Mirhafez
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  10. Gordon A. Ferns
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  11. Maryam Mardan-Nik
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  12. Elham Mohammadzadeh
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  13. Amir Avan
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  14. Parichehr Hanachi
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  15. Majid Ghayour-Mobarhan
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Corresponding authors

Correspondence to Latiffah A. Latiff or Majid Ghayour-Mobarhan.

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Tajfard, M., Latiff, L.A., Rahimi, H.R. et al. Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography. Mol Cell Biochem 435, 37–45 (2017). https://doi.org/10.1007/s11010-017-3054-5

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  • DOI: https://doi.org/10.1007/s11010-017-3054-5

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