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Kinetics of expression of genes involved in glucose metabolism after the last meal in overfed mule ducks

Molecular and Cellular Biochemistry volume 430, pages 127–137 (2017)Cite this article

Abstract

In waterfowls, overfeeding leads to a hepatic steatosis, also called “foie gras.” We decided to investigate the role of glucose metabolism in steatosis emergence. For this, we measured the expression of genes during the 12 h following the last meal of the overfeeding period. As expected, it showed that the expression of glucose transporter is more precocious in jejunal mucosa, especially for SGLT1, known to be the major transporter at the apical surface. In the liver, GLUT2 and HK1 are upregulated at the same time and seem to work together to import glucose. In peripherals tissues, such as muscle and subcutaneous adipose tissue (SAT), expression of genes of interest occurs later than the one in jejunum and liver. These results are in accordance with the evolution of glycemia. This study allows us to better understand the kinetic treatment of glucose after a meal in overfed ducks. It also will allow researchers to better target their sampling time knowing the optimal point of expression of each gene.

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Acknowledgements

We thank the “Conseil Général des Landes” and the “Comité Interprofessionnel des Palmipèdes à Foie Gras” (CIFOG) for financing this work. We also thank the technical staff of INRA Artiguères for rearing ducks (Certificate of Authorization to Experiment on Living animals, No. B40-037-1, Ministry of Agriculture and Fish Products, ethic committee Aquitaine birds and fish No.C2EA-73). We are grateful to Frédéric Martins and Jean-José Maoret for performing Fluidigm analysis (Génopole Toulouse/Midi-pyrénées, Plateau Transcriptomique Quantitative (TQ), Toulouse, France). We finally thank Patrick Daniel, Karine Bellet, and Martine Chague of the laboratory Pyrénées Landes (LPL, Mont de Marsan) to let us use their materials.

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Affiliations

  1. UMR 1419 INRA UPPA NuMéA, 371 Rue du Ruisseau, 40000, Mont de Marsan, France

    Tavernier Annabelle, Ricaud Karine, Davail Stéphane & Gontier Karine

  2. UMR 1419 INRA UPPA NuMéA, Quartier Ibarron, 64310, Saint Pée sur Nivelle, France

    Tavernier Annabelle, Ricaud Karine, Davail Stéphane & Gontier Karine

  3. INRA UEPFG, 1076 Route de Haut Maucq, 40280, Benquet, France

    Bernadet Marie-Dominique

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  1. Tavernier Annabelle
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  2. Ricaud Karine
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  3. Bernadet Marie-Dominique
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  4. Davail Stéphane
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Correspondence to Gontier Karine.

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Annabelle, T., Karine, R., Marie-Dominique, B. et al. Kinetics of expression of genes involved in glucose metabolism after the last meal in overfed mule ducks. Mol Cell Biochem 430, 127–137 (2017). https://doi.org/10.1007/s11010-017-2960-x

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  • DOI: https://doi.org/10.1007/s11010-017-2960-x

Keywords

  • Hepatic steatosis
  • Glucose metabolism
  • Kinetic
  • Ducks