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Regulation of PPARγ and CIDEC expression by adenovirus 36 in adipocyte differentiation

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Abstract

This study is to investigate the role of adenovirus 36 (Ad36) in regulating expression of peroxisome proliferator-activated receptor γ (PPARγ) and cell death-inducing DFFA-like effector c (CIDEC) in Ad36-induced adipocyte differentiation. Human adipose-derived mesenchymal stem cells (hAMSCs) were isolated and cultured, and then infected with Ad36. Ad36-induced adipocytes were identified using quantitative real-time PCR and Oil red O staining. The expression levels of PPARγ and CIDEC in Ad36-induced adipocytes were determined by quantitative real-time PCR and Western blot analysis. Glucose uptake and intracellular triglyceride content were also determined in these induced cells. Our results from the Oil red O staining showed that Ad36 induced the differentiation of hAMSCs into human adipocytes in vitro. Moreover, the medium glucose concentration was significantly decreased, while the intracellular triglyceride content was significantly increased, in the Ad36-induced adipocytes, compared with the control group. Furthermore, our results showed that, the mRNA and protein expression levels of PPARγ and CIDEC were significantly upregulated in Ad36-induced adipocytes, in a time-dependent manner. On the other hand, compared with the control group, the CIDEC expression was downregulated when the Ad36-induced adipocytes were treated with the PPARγ inhibitor, GW9662. Ad36 could upregulate the expression level of CIDEC through increasing PPARγ expression during the adipocyte differentiation process.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 81660154) and the College Students Innovation Experiment Program (CX2015002).

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    Authors and Affiliations

    1. Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China

      Yi Jiao, Yiliyasi Aisa, Xiaodi Liang, Nuerbiye Nuermaimaiti, Xian Gong & Yaqun Guan

    2. Medical Testing Center, The First Affiliated Hospital of Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China

      Zhaoxia Zhang

    3. Diabetes Center, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, 830011, Xinjiang, China

      Yaqun Guan

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    1. Yi Jiao
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    2. Yiliyasi Aisa
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    5. Xian Gong
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    Correspondence to Zhaoxia Zhang or Yaqun Guan.

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    All authors declare no financial conflict of interest. All authors declare no non-financial conflict of interest.

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    Yi Jiao and Yiliyasi Aisa contributed equally to this work.

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    Jiao, Y., Aisa, Y., Liang, X. et al. Regulation of PPARγ and CIDEC expression by adenovirus 36 in adipocyte differentiation. Mol Cell Biochem 428, 1–8 (2017). https://doi.org/10.1007/s11010-016-2912-x

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    • DOI: https://doi.org/10.1007/s11010-016-2912-x

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