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Dodeca-2(E),4(E)-dienoic acid isobutylamide enhances glucose uptake in 3T3-L1 cells via activation of Akt signaling

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Abstract

Dodeca-2(E),4(E)-dienoic acid isobutylamide (DDI), an alkamide derived from the plant Echinacea purpurea, promotes adipocyte differentiation and activates peroxisome proliferator-activated receptor γ, which is associated with enhanced insulin sensitivity. In the present study, we investigated whether DDI may increase glucose uptake through activation of the insulin signaling pathway in 3T3-L1 adipocytes. DDI increased insulin-stimulated glucose uptake, and expression and translocation of glucose transporter 4 in adipocytes treated with sub-optimal levels of insulin. Additionally, DDI enhanced Akt phosphorylation, whereas phosphoinositide 3-kinase/Akt inhibitors suppressed DDI-induced glucose uptake. These results suggest that DDI may improve insulin sensitivity through the activation of Akt signaling, which leads to enhanced glucose uptake.

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Abbreviations

DDI:

Dodeca-2(E),4(E)-dienoic acid isobutylamide

IRS:

Insulin receptor substrate

PI3K:

Phosphoinositide 3-kinase

GLUT4:

Glucose transporter 4

PPARγ:

Peroxisome proliferator-activated receptor γ

DMEM:

Dulbecco’s Modified Eagle’s Medium

BCS:

Bovine calf serum

FBS:

Fetal bovine serum

BSA:

Bovine serum albumin

IBMX:

3-Isobutyl-1-methylxanthine

PBS:

Phosphate-buffered saline

KRH:

Krebs–Ringer Hepes

SD:

Standard deviation

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Acknowledgements

This research was supported by the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-Industry Convergence (BAIO), the National Research Foundation of Korea [NRF] grant funded by the Korea government (MSIP) (No. MRC, 2008-0062275) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MOE) (No. 2015R1D1A1A01060790).

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  1. College of Pharmacy, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, Republic of Korea

    Kyeong-Mi Choi, Wonkyun Kim, Jin Tae Hong & Hwan-Soo Yoo

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  1. Kyeong-Mi Choi
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Correspondence to Hwan-Soo Yoo.

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Choi, KM., Kim, W., Hong, J.T. et al. Dodeca-2(E),4(E)-dienoic acid isobutylamide enhances glucose uptake in 3T3-L1 cells via activation of Akt signaling. Mol Cell Biochem 426, 9–15 (2017). https://doi.org/10.1007/s11010-016-2876-x

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