Skip to main content
SpringerLink
Log in

Baculovirus antiapoptotic protein P35 regulated the host apoptosis to enhance virus multiplication

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) possesses p35 gene, which encodes antiapoptosis protein P35 (or Ac-P35). A previous study showed that deleting p35 promoted premature cytolysis and caused the reduction of virus yield. In this report, we examined the role of P35 in regulating the expression of Ac-IAPs (inhibitor of apoptosis proteins in AcMNPV) and SfP53 (an apoptosis protein in Sf9 cells), and its effect on the production of progeny virus. Results showed that the overexpression of P35 caused a delay in the increase process of SfP53 before 36-h post infection and improved the transcription levels of iaps gene dramatically; it was more favorable to improve the transcription level of iap1 at 24–72 h post infection. Moreover, P35 could promote the production of progeny virus. This is the first study to disclose the relationship among p35, iap1, iap2, Sfp53 and the replication of the virus in the AcMNPV infection process, which provides the basis for improving the insecticidal activity of recombinant AcMNPV in terms of theory and technology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Ayres MD, Howard SC, Kuzio J, Lopez-Ferber M, Possee RD (1994) The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605

    Article  CAS  PubMed  Google Scholar 

  2. Herniou EA, Olszewski JA, Cory JS, O’Reilly DR (2003) The genome sequence and evolution of baculoviruses. Annu Rev Entomol 48:211–234

    Article  CAS  PubMed  Google Scholar 

  3. Jakubowska AK, Peters SA, Ziemnicka J, Vlak JM (2006) Genome sequence of an enhancin gene-rich nucleopolyhedrovirus (NPV) from Agrotis segetum: collinearity with Spodoptera exigua multiple NPV. J Gen Virol 87:537–551

    Article  CAS  PubMed  Google Scholar 

  4. Tao XY, Choi JY, Kim WJ, Lee JH, Liu Q, Kim SE (2013) The Autographa californica multiple nucleopolyhedrovirus ORF78 is essential for budded virus production and general occlusion body formation. J Virol 87:8441–8450

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Clem RJ (2015) Viral IAPs, then and now. Semin Cell Dev Biol 39:72–79

    Article  CAS  PubMed  Google Scholar 

  6. Rohrmann GF (2013) The AcMNPV genome: gene content, conservation and function. In: Baculovirus molecularbiology, National Library of Medicine (US), National Center for Biotechnology Information, Bethesda, p 8

  7. Qian Y, Xiong YH, Liu JL, Wang Q (2016) Comparative proteomics analysis of apoptotic Spodoptera frugiperda cells during p35 knockout Autographa californica multiple nucleopolyhedrovirus infection. Comp Biochem Physiol Part D 18:21–29

    Google Scholar 

  8. LaCount DJ, Hanson SF, Schneider CL (2000) Caspase inhibitor p35 and inhibitor of apoptosis Op-IAP block in vivo proteolytic activation of an effector caspase at different steps. J Biol Chem 275:15657–15664

    Article  CAS  PubMed  Google Scholar 

  9. Clem RJ, Miller LK (1994) Control of programmed cell death by the baculovirus genes p35 and iap. Mol Cell Biol 14:5212–5222

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Braunagel SC, Daniel KD, Reilly LM, Guarino LA (1992) Sequence, genomic organisation of the EcoRIA fragment of Autographa californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of rotavirus. Virology 191:1003–1008

    Article  CAS  PubMed  Google Scholar 

  11. Crook NE, Clem RJ, Miller LK (1993) An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. J Virol 67:2168–2174

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Griffiths CM, Barnett AL, Ayres MD, Windass J, King LA (1999) In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2. J Gen Virol 80:1055–1066

    Article  CAS  PubMed  Google Scholar 

  13. Zeng XD, Nan F, Song JH, Wang Q (2009) Functional analysis of the Autographa californica nucleopolyhedrovirus IAP1 and IAP2. Sci China Ser C Life Sci 52:761–770

    Article  Google Scholar 

  14. Ikeda M, Yamada H, Ito H, Kobayashi M (2011) Baculovirus IAP1 induces caspase-dependent apoptosis in insect cells. J Gen Virol 92:2654–2663

    Article  CAS  PubMed  Google Scholar 

  15. Huang N, Clem RJ, Rohrmann GF (2011) Characterization of cDNAs encoding p53 of Bombyx mori and Spodoptera frugiperda. Insect Biochem Mol Biol 41:613–619

    Article  CAS  PubMed  Google Scholar 

  16. Huang N, Wu W, Yang K, Passarelli AL, Rohrmann GF, Clem RJ (2011) Baculovirus infection induces a DNA damage response that is required for efficient viral replication. J Virol 85:12547–12556

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Mehrabadi M, Hussain M, Matindoost L, Asgari S (2015) The baculovirus antiapoptotic p35 protein functions as an inhibitor of the host RNA interference antiviral response. J Virol 89:8182–8192

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Wu CX, Wang S (2012) A pH-sensitive heparin-binding sequence from baculovirus gp64 protein is important for binding to mammalian cells but not to Sf9 insect cells. J Virol 86:484–491

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Fu YJ, Lin TT, Liang AH, Hu FY (2016) Effects of recombinant baculovirus AcMNPV-BmK IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells. Cytotechnology 68:381–387

    Article  CAS  PubMed  Google Scholar 

  20. Fu YJ, Zhao J, Liang AH, Hu FY (2015) AcMNPV-mediated expression of BmK IT promotes the apoptosis of Sf9 cells and replication of AcMNPV. Acta Physiol Sinica 67:305–311

    CAS  PubMed  Google Scholar 

  21. Lee JC, Chen HH, Chao YC (1998) Persistent baculovirus infection results from deletion of the apoptotic suppressor gene p35. J Virol 72:9157–9165

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Hershberger PA, LaCount DJ, Friesen PD (1994) The apoptotic suppressor P35 is required early during baculovirus replication and is targeted to the cytosol of infected cells. J Virol 68:3467–3477

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Kool M, Ahrens CH, Goldbach RW, Rohrmann GF, Vlak JM (1994) Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proc Natl Acad Sci USA 91:11212–11216

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Lung O, Westenberg M, Vlak JM, Zuidema D, Blissard GW (2002) Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): f proteins from group II NPVs are functionally analogous to AcMNPV GP64. J Virol 76:5729–5736

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Fu YJ, Miao Y, Liang AH, Zheng SH (2016) AcMNPV-BmK IT improves the progeny virus production via baculovirus GP64 envelope fusion protein. Biotechnol Lett 38:1673–1681. doi:10.1007/s10529-016-2146-8

    Article  CAS  PubMed  Google Scholar 

  26. Zhang P, Yang K, Dai X (2002) Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae. J Gen Virol 83:3003–3011

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This project was supported by Grants from ‘National Natural Science Foundation of China (No. 31272100 and 31372199)’ and ‘Natural Science Foundation of Shanxi Province (No. 2014011038-1)’.

Author information

Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Yanyan Miao, Aihua Liang & Yuejun Fu

Authors
  1. Yanyan Miao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aihua Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuejun Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuejun Fu.

Ethics declarations

Conflict of interest

The authors state that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Miao, Y., Liang, A. & Fu, Y. Baculovirus antiapoptotic protein P35 regulated the host apoptosis to enhance virus multiplication. Mol Cell Biochem 423, 67–73 (2016). https://doi.org/10.1007/s11010-016-2825-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-016-2825-8

Keywords

Search

Navigation