Molecular and Cellular Biochemistry

, Volume 412, Issue 1–2, pp 235–245 | Cite as

Overexpression of miR-335 confers cell proliferation and tumour growth to colorectal carcinoma cells

  • Yanxia Lu
  • Hui Yang
  • Li Yuan
  • Guobing Liu
  • Chao Zhang
  • Min Hong
  • Yan Liu
  • Min Zhou
  • Fang Chen
  • Xuenong Li
Article

Abstract

The involvement of miR-335 in csolorectal cancer (CRC) development remains controversial. Here, we found that miR-335 was highly up-regulated in CRC specimens relative to normal mucosa, and high miR-335 expression level was markedly associated with the tumour size and differentiation of CRC. The overexpression of miR-335 in CRC cells facilitated cell proliferation in vitro and tumour growth in vivo. RASA1 was validated as a target of miR-335 that was downregulation in CRC. Forced expression of miR-335 silenced RASA1 and triggered Ras/ERK cascade in CRC. Together, miR-335-RASA1 contributes to cell growth in CRC, and elucidation of downstream pathway will provide new insights into the molecular mechanisms of CRC progression.

Keywords

miR-335 Colorectal carcinoma RASA1 Proliferation Tumour growth 

Abbreviations

CRC

Colorectal carcinoma

miRNA

microRNA

UTR

Untranslated regions

RASA1

RAS p21 protein activator 1

SD

Standard deviation

CCK-8

Cell counting kit-8

GFP

Green fluorescent protein

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yanxia Lu
    • 1
  • Hui Yang
    • 1
    • 2
  • Li Yuan
    • 1
  • Guobing Liu
    • 3
  • Chao Zhang
    • 1
    • 4
  • Min Hong
    • 1
  • Yan Liu
    • 1
  • Min Zhou
    • 1
  • Fang Chen
    • 1
  • Xuenong Li
    • 1
  1. 1.Department of Pathology, School of Basic Medical SciencesSouthern Medical UniversityGuangzhouChina
  2. 2.Department of PathologyXi’an 141 HospitalXi’anChina
  3. 3.Department of Obstetrics and Gynecology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  4. 4.Department of PathologySun Yat-Sen University Cancer CenterGuangzhouChina

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