Abstract
The aim of this study was to assess the role of nitric oxide (NO) in cardiac anaphylaxis regarding changes in coronary reactivity and oxidative status of the mice heart. The animals were divided into two groups: experimental group (CBA, iNOS−/− mice) and control group: wild-type mice (CBA/H). The hearts of male mice (n = 24; 6–8 weeks old, body mass 20–25 g, 12 in each experimental group) were excised and retrogradely perfused according to the Langendorff technique at a constant perfusion pressure (70 cm H2O). Cardiac anaphylaxis was elicited by injection of solution (1 mg/1 ml) of ovalbumin into the aortic cannula. For the next 10 min, in intervals of 2 min (0–2, 2–4, 4–6, 6–8, 8–10 min) coronary flow (CF) rates were measured and samples of coronary effluent were collected. Markers of oxidative stress including index of lipid peroxidation measured as thiobarbituric acid-reactive substances (TBARS), NO measured in the form of nitrites (\({\text{NO}}_{2}^{ - }\)), superoxide anion radical (\({\text{O}}_{2}^{ - }\)), and hydrogen peroxide (H2O2) in the coronary venous effluent were assessed spectrophotometrically. After the ovalbumin challenge, CF was significantly lower in the wild mice group. NO and H2O2 release were significantly higher in iNOS−/− mice group. TBARS and \({\text{O}}_{2}^{ - }\) values did not vary significantly between wild and iNOS−/− mice groups. Our results indicate that coronary vasoconstriction during cardiac anaphylaxis does not necessarily depend on inducible nitric oxide synthase (iNOS)/NO activity and that iNOS/NO pathway may not be an only influential mediator of redox changes in this model of cardiac anaphylaxis.
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Abbreviations
- CF:
-
Coronary flow
- eNOS:
-
Endothelial nitric oxide synthase
- HRPO:
-
Peroxidase from horse radish
- iNOS:
-
Inducible nitric oxide synthase
- IgE:
-
Immunoglobulin E
- NBT:
-
Nitro blue tetrazolium
- NO:
-
Nitric oxide
- PRS:
-
Phenol red solution
- PAF:
-
Platelet-activating factor
- ROS:
-
Reactive oxygen species
- TBARS:
-
Thiobarbituric acid-reactive substances
- TBA:
-
Thiobarbituric acid
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Acknowledgments
This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 175043), and the Faculty of Medical Sciences, University of Kragujevac (Junior Project 04/11).
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Milicic, V., Zivkovic, V., Jeremic, N. et al. Coronary flow and oxidative stress during local anaphylactic reaction in isolated mice heart: the role of nitric oxide (NO). Mol Cell Biochem 412, 221–227 (2016). https://doi.org/10.1007/s11010-015-2628-3
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DOI: https://doi.org/10.1007/s11010-015-2628-3