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The Sub1 nuclear protein protects DNA from oxidative damage

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Abstract

Reactive oxygen species are a by-product of aerobic metabolism that can damage lipid, proteins, and nucleic acids. Oxidative damage to DNA is especially critical, because it can lead to cell death or mutagenesis. Previously we reported that the yeast sub1 deletion mutant is sensitive to hydrogen peroxide treatment and that the human SUB1 can complement the sensitivity of the yeast sub1 mutant. In this study, we find that Sub1 protects DNA from oxidative damage in vivo and in vitro. We demonstrate that transcription of SUB1 mRNA is induced by oxidative stress and that the sub1Δ mutant has an increased number of chromosomal DNA strand breaks after peroxide treatment. We further demonstrate that purified Sub1 protein can protect DNA from oxidative damage in vitro, using the metal ion catalyzed oxidation assay.

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Acknowledgments

We thank Michael Hampsey, James Haber, and Johannes Hegemann for yeast strains and plasmids, Kenan Murphy and Jen-Yeu Wang for strain construction and Martin Marinus, Anita Fenton, Feng He, Yahui Kong, and Hang Cui for technical advice and assistance. This work was funded in part by NIH grant CA100122.

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Authors and Affiliations

  1. Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA, 01655, USA

    Lijian Yu, Hong Ma & Michael R. Volkert

  2. The Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA, USA

    Xincai Ji

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  1. Lijian Yu
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  2. Hong Ma
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  3. Xincai Ji
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  4. Michael R. Volkert
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Correspondence to Michael R. Volkert.

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Yu, L., Ma, H., Ji, X. et al. The Sub1 nuclear protein protects DNA from oxidative damage. Mol Cell Biochem 412, 165–171 (2016). https://doi.org/10.1007/s11010-015-2621-x

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  • DOI: https://doi.org/10.1007/s11010-015-2621-x

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