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Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression

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Abstract

The activated renin–angiotensin–aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.

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Acknowledgments

This study was supported by research grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—2009/07671-2), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (475586/2009-3), and INCT-Nano-Biofarmacêutica

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Authors and Affiliations

  1. Laboratório de Bioquímica e Biologia Molecular, Instituto de Ciências Exatas e Biológicas, Núcleo de Pesquisa em Biologia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil

    Walmir da Silva

  2. Laboratório de Fisiologia, Departmento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Robson Augusto Souza dos Santos

  3. Laboratório de Biologia Molecular, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Rio Claro, SP, 13506-900, Brazil

    Karen C. M. Moraes

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  1. Walmir da Silva
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  2. Robson Augusto Souza dos Santos
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  3. Karen C. M. Moraes
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Correspondence to Karen C. M. Moraes.

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da Silva, W., dos Santos, R.A.S. & Moraes, K.C.M. Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression. Mol Cell Biochem 411, 363–371 (2016). https://doi.org/10.1007/s11010-015-2598-5

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  • DOI: https://doi.org/10.1007/s11010-015-2598-5

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