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Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells

Molecular and Cellular Biochemistry volume 411, pages 1–10 (2016)Cite this article

Abstract

Immune cells and inflammatory mediators play important roles in the development of atherosclerotic vascular inflammation. IL-27 is a member of the IL-6/IL-12 family that can promote Th1 responses and augment the release of inflammatory mediators from human mast cells and monocytes. However, the direct effect of IL-27 on human coronary artery endothelial cells was unclear. In this study, the effects of IL-27 and TNF-α on the cell surface expression of adhesion molecules, inflammatory cytokines, and chemokines were investigated. Results showed that IL-27 alone could significantly promote the release of chemokine CXCL10. However, IL-27 could further significantly enhance the TNF-α-mediated upregulation of adhesion molecules ICAM-1 and VCAM-1, inflammatory cytokine IL-6, as well as chemokines CCL5 and CXCL10 from human coronary artery endothelial cells. The release of IL-6, CCL5, and CXCL10 were significantly suppressed by specific signaling molecule inhibitors, implying that the induction of these mediators from the human coronary artery endothelial cells could be differentially regulated by the c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB pathways. These results provided new insights into the effect of IL-27 on the TNF-α mediated activation of human coronary artery endothelial cells in atherosclerotic vascular inflammation.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Project No. 81401309).

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Affiliations

  1. Guangzhou Institute of Cardiovascular Disease, The Second Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong Province, China

    Huai-Na Qiu, Bin Liu, Weihua Liu & Shiming Liu

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  1. Huai-Na Qiu
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  2. Bin Liu
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  3. Weihua Liu
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  4. Shiming Liu
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Correspondence to Shiming Liu.

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Qiu, HN., Liu, B., Liu, W. et al. Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells. Mol Cell Biochem 411, 1–10 (2016). https://doi.org/10.1007/s11010-015-2563-3

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  • DOI: https://doi.org/10.1007/s11010-015-2563-3

Keywords

  • Vascular inflammation
  • Cytokine
  • Chemokine
  • HCAEC