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Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development

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Abstract

SRY-related box (Sox) transcription factors are conserved among vertebrate species. These proteins regulate multiple processes including sex determination and testis differentiation of the male embryo. Although members of the Sox family have been identified in pre- and postnatal Sertoli cells, they have never been characterized in adult Leydig cells. The objectives of this research were to identify expressions of Sox9, Sox5, and Sox13 in mice Leydig cell cultures and to establish their expression profiles in postnatal mice testes at different developmental stages. Methods used include Western blots and qPCR of stimulated MA-10 cell cultures and whole mice testes. Sox9, Sox5, and Sox13 proteins were detected in MA-10 cells as well as whole mouse testis. Although Sox9, Sox5, and Sox13 mRNA levels from whole mice testes tended to increase according to postnatal development, these results were not significant. Sox members were also detected in whole mice testis by Western Blot. However, Sox9, Sox5, and Sox13 protein expressions remained relatively constant during postnatal development from postnatal (P) day 60 to P365. Being newly characterized in the mouse testis, Sox13 was mainly localized by immunofluorescence within the nuclei of cells from seminiferous tubules, possibly spermatocytes and Sertoli cells. In addition, Sox9, Sox5, and Sox13 proteins were characterized in the nuclei of MA-10 Leydig cell cultures. Their expressions and transcriptional activities remained unaffected by activators of the cAMP/PKA pathway. Thus, Sox9, Sox5, and Sox13 transcription factors are expressed in postnatal testis and may regulate multiple functions such as steroidogenesis and spermatogenesis.

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Acknowledgments

We would like to thank Dr. Mario Ascoli for generously providing the MA-10 cell line used in this study. This work was supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada (#386557-2012 to L.J.M.) and the New Brunswick Innovation Foundation (NBIF) (#IAR2012 and IAR2013-029 to L.J.M.).

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The authors declare that there is no conflict of interest that would prejudice there impartiality.

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    1. Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, Moncton, NB, E1A 3E9, Canada

      Mikella Daigle, Pauline Roumaud & Luc J. Martin

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    Mikella Daigle and Pauline Roumaud have contributed equally to this work.

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    Daigle, M., Roumaud, P. & Martin, L.J. Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development. Mol Cell Biochem 407, 209–221 (2015). https://doi.org/10.1007/s11010-015-2470-7

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