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Molecular and Cellular Biochemistry

, Volume 406, Issue 1–2, pp 9–20 | Cite as

Hesperidin ameliorates trichloroethylene-induced nephrotoxicity by abrogation of oxidative stress and apoptosis in wistar rats

  • Aisha Siddiqi
  • Sana Nafees
  • Summya Rashid
  • Sarwat SultanaEmail author
  • Bano Saidullah
Article

Abstract

Trichloroethylene (TCE), a nephrotoxicant is known to cause severe damage to the kidney. In this study, the nephroprotective potential of hesperidin was evaluated against TCE-induced nephrotoxicity in wistar rats. Oral administration of TCE (1000 mg/kg b.wt) for 15 days enhanced renal lipid peroxidation and reduced antioxidant enzymes armoury viz., reduced renal glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, catalase and superoxide dismutase. It also enhanced the levels of blood urea nitrogen, creatinine and kidney injury molecule (KIM-1). Caspase-3 and bax expression were found to be elevated, while that of bcl-2 reduced suggesting that TCE induces apoptosis. However, pretreatment with hesperidin at a dose of 100 and 200 mg/kg b.wt for 15 days significantly decreased lipid peroxidation, increased the levels of antioxidant enzymes and reduced blood urea, creatinine and KIM-1 levels. Hesperidin also modulated the apoptotic pathways by altering the expressions of caspase-3, bax and bcl-2 to normal. Our results suggest that hesperidin can be used as a nephroprotective agent against TCE-induced nephrotoxicity.

Graphical Abstract

Keywords

Trichloroethylene Nephrotoxicity Hesperidin Oxidative stress Apoptosis 

Abbreviations

TCE

1,1,2-Trichloroethylene

DCVG

S-(1,2-dichlorovinyl)-l-glutathione

DCVC

S-(1,2-dichlorovinyl)-l-cysteine

GPx

Glutathione peroxidase

GSH

Glutathione

GR

Glutathione reductase

GST

Glutathione-S-transferase

LPO

Lipid peroxidation

MDA

Malondialdehyde

PMS

Post-mitochondrial supernatant

SOD

Superoxide dismutase

BUN

Blood urea nitrogen

KIM-1

Kidney injury molecule

HS

Hesperidin

ROS

Reactive oxygen species

Notes

Acknowledgments

The author (SS), Jamia Hamdard University acknowledge the help given to the student (AS) for getting registered to PhD in Indira Gandhi National Open University, New Delhi, India.

Conflict of interest

The authors of the present research work do not have any conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Aisha Siddiqi
    • 2
  • Sana Nafees
    • 1
  • Summya Rashid
    • 1
  • Sarwat Sultana
    • 1
    Email author
  • Bano Saidullah
    • 2
  1. 1.Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of ScienceJamia Hamdard (Hamdard University)New DelhiIndia
  2. 2.Discipline of Life Sciences, School of SciencesIndira Gandhi National Open UniversityNew DelhiIndia

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