Molecular and Cellular Biochemistry

, Volume 390, Issue 1, pp 69–74

miR-143 suppresses osteogenic differentiation by targeting Osterix

Article

DOI: 10.1007/s11010-013-1957-3

Cite this article as:
Li, E., Zhang, J., Yuan, T. et al. Mol Cell Biochem (2014) 390: 69. doi:10.1007/s11010-013-1957-3

Abstract

Osterix (Osx) is an essential transcription factor for osteoblast differentiation and bone formation. However, the knowledge of the regulation of Osx expression is poor. MicroRNAs (miRNAs), a class of small non-coding RNAs, play critical roles in numerous biological processes, including the proliferation, differentiation, and survival of cells and organisms. Herein, we aimed to explore the effect of miR-143 on Osx expression and osteogenic differentiation. miR-143, which was suppressor of the osteogenic differentiation of MC3T3-E1 cells, had decreased levels of expression during osteogenic differentiation. Moreover, Osx was identified to be a direct target of miR-143. Inhibition of Osx performed similar effect with miR-143 on osteogenic differentiation, while overexpression of Osx could partially reverse the suppressive effect of miR-143. Collectively, these data indicate that miR-143 is a novel regulator of Osx, and it might play an essential role in the regulation of osteogenic differentiation.

Keywords

miR-143 Osteogenesis Differentiation Osterix 

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Enqi Li
    • 1
  • Jinli Zhang
    • 1
  • Tianxiang Yuan
    • 1
  • Baotong Ma
    • 1
  1. 1.Department of Orthopaedic TraumaTianJin HospitalTianjinChina

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