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Silencing of TBC1D15 promotes RhoA activation and membrane blebbing

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Abstract

Membrane blebs are round-shaped dynamic membrane protrusions that occur under many physiological conditions. Membrane bleb production is primarily controlled by actin cytoskeletal rearrangements mediated by RhoA. Tre2–Bub2–Cdc16 (TBC) domain-containing proteins are negative regulators of the Rab family of small GTPases and contain a highly conserved TBC domain. In this report, we show that the expression of TBC1D15 is associated with the activity of RhoA and the production of membrane blebs. Depletion of TBC1D15 induced activation of RhoA and membrane blebbing, which was abolished by the addition of an inhibitor for RhoA signaling. In addition, we show that TBC1D15 is required for the accumulation of RhoA at the equatorial cortex for the ingression of the cytokinetic furrow during cytokinesis. Our results demonstrate a novel role for TBC1D15 in the regulation of RhoA during membrane blebbing and cytokinesis.

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Acknowledgments

We would like to thank the members of the Division of Cancer Biology for helpful discussions and technical assistances. This research was partially funded by a grant from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Yuko Takahara, Masao Maeda, Hitoki Hasegawa, Satoko Ito, Toshinori Hyodo, Eri Asano, Michinari Hamaguchi & Takeshi Senga

  2. Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Masahide Takahashi

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  1. Yuko Takahara
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  2. Masao Maeda
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Correspondence to Takeshi Senga.

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Takahara, Y., Maeda, M., Hasegawa, H. et al. Silencing of TBC1D15 promotes RhoA activation and membrane blebbing. Mol Cell Biochem 389, 9–16 (2014). https://doi.org/10.1007/s11010-013-1921-2

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  • DOI: https://doi.org/10.1007/s11010-013-1921-2

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