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Activation of prosurvival signaling pathways during the memory phase of volatile anesthetic preconditioning in human myocardium: a pilot study

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Abstract

According to a compelling body of evidence anesthetic preconditioning (APC) attenuates the deleterious consequences of ischemia–reperfusion and protects the heart through a mechanism similar to ischemic preconditioning. The present study was purported to investigate the intracellular signaling pathways activated in human myocardium in response to a preconditioning protocol with two different volatile anesthetics, namely isoflurane and sevoflurane. To this aim, phosphorylation of PKCα and -δ, ERK1/2, Akt, and GSK3β was determined at the end of the APC protocol, in human atrial samples harvested from patients undergoing open-heart surgery. The results demonstrate that preconditioning with volatile anesthetics triggers the activation of PKCδ and -α isoforms and of prosurvival kinases, ERK1/2, and Akt, while inhibiting their downstream target GSK3β during the memory phase.

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Acknowledgments

Technical support of Drs. Radu Scarlat and Cristian Boros is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Animal Physiology, School of Biology, Aristotle University of Thessaloniki, Thessaloníki, Greece

    Kyriakos Mellidis, Eleftheria Galatou & Antigone Lazou

  2. Department of Pathophysiology, “Victor Babeş” University of Medicine and Pharmacy of Timişoara, Timisoara, Romania

    Valentin Ordodi, Oana Duicu & Danina Muntean

  3. Department of Anesthesiology and Intensive Care, “Victor Babeş” University of Medicine and Pharmacy of Timişoara, Timisoara, Romania

    Dorel Săndesc

  4. Cardiology 1 – Intensive Care Unit, “C.C.Iliescu” Institute of Emergency for Cardiovascular Diseases, Bucharest, Romania

    Şerban Bubenek

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  1. Kyriakos Mellidis
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  2. Valentin Ordodi
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  3. Eleftheria Galatou
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Correspondence to Danina Muntean.

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Kyriakos Mellidis and Valentin Ordodi have contributed equally to this work.

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Mellidis, K., Ordodi, V., Galatou, E. et al. Activation of prosurvival signaling pathways during the memory phase of volatile anesthetic preconditioning in human myocardium: a pilot study. Mol Cell Biochem 388, 195–201 (2014). https://doi.org/10.1007/s11010-013-1910-5

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  • DOI: https://doi.org/10.1007/s11010-013-1910-5

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