Molecular and Cellular Biochemistry

, Volume 384, Issue 1–2, pp 147–153 | Cite as

Anticancer potential of rhamnocitrin 4′-β-d-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats

  • Shakir Saleem
  • Md Adil Shaharyar
  • Mohammad Jawed Khusroo
  • Parwej Ahmad
  • Rais Ur Rahman
  • Kamran Ahmad
  • Md Jahangir Alam
  • Naif O Al-Harbi
  • Muzaffar Iqbal
  • Faisal Imam
Article
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Abstract

The hepatoprotective activity of flavonoid rhamnocitrin 4′-β-d-galactopyranoside (RGP) obtained from leaves of Astragalus hamosus L. against N-diethylnitrosamine (DENA)-induced hepatic cancer in Wistar albino rats was evaluated. Hepatic cancer in rats was induced by single-dose intraperitoneal administration of DENA (200 mg/kg). Induction of hepatic cancer was confirmed after 7 days of DENA administration by measurement of elevated level of serum α-feto protein (AFP). Administration of DENA in a single dose lofted the levels of serum biochemical parameters like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total protein and AFP. Antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and lipid per oxidation (LPO) were annealed significantly by administration of RGP in a dose-dependant manner. The histopathological examination of rat liver section was found to reinforce the biochemical observations significantly. It was observed that a substantial and dose-dependent reversal of DENA-diminished activity of antioxidant enzymes like SOD, CAT, GPx, GST and the reduced DENA-elevated level of LPO with a marked change. Any elevation in the levels of serum markers along with suppression of free radical formation by scavenging the hydroxyl radicals is significantly prevented by RGP. It also modulates the levels of LPO and perceptibly increases the endogenous antioxidant enzymes level in DENA-induced hepatocellular carcinogenesis. The findings suggest that RGP prevents hepatocellular carcinoma by suppressing the marked increase in the levels of serum marker enzymes, and suppresses the free radical by scavenging hydroxyl radicals.

Keywords

DENA Hepatocellular carcinoma Rhamnocitrin 4′-β-d-galactopyranoside Biochemical parameters Anticancer 

Notes

Acknowledgments

The authors would like to extend their sincere appreciation to the deanship of scientific research at the King Saud University for its funding of this research through the research Group Project No. RGP-VPP-305.

Disclosure

None.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Shakir Saleem
    • 1
  • Md Adil Shaharyar
    • 1
  • Mohammad Jawed Khusroo
    • 2
  • Parwej Ahmad
    • 3
  • Rais Ur Rahman
    • 7
  • Kamran Ahmad
    • 1
  • Md Jahangir Alam
    • 4
  • Naif O Al-Harbi
    • 6
  • Muzaffar Iqbal
    • 5
  • Faisal Imam
    • 6
  1. 1.Department of PharmacologyLuqman College of PharmacyGulbargaIndia
  2. 2.Department of Pharmacology, Faculty of PharmacyJamia HamdardNew DelhiIndia
  3. 3.Faculty of UnaniJamia HamdardNew DelhiIndia
  4. 4.Department of Pharmaceutical ChemistryJamia HamdardNew DelhiIndia
  5. 5.Department of Pharmaceutical Chemistry, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  6. 6.Department of Pharmacology and Toxicology, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  7. 7.Sri Siddhartha Medical College and Research CentreTumkurIndia

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