Abstract
Heat shock protein 27 (Hsp27) can regulate actin cytoskeleton dynamics and contractile protein activation. This study investigates whether Hsp27 expression is related to bladder contractile dysfunction after acute urinary retention (AUR). Female rats were randomized either to AUR by urethral ligation or to normal control group. Bladder and smooth muscle strip contraction at time points from 0 h to 7 days after AUR were estimated by cystometric and organ bath studies. Hsp27 expression in bladder tissue at each time point was detected with immunofluorescence, Western blots, and real-time PCR. Expression of the three phosphorylated forms of Hsp27 was detected by Western blots. Smooth muscle ultrastructure was observed by transmission electron microscopy. Data suggest that maximum detrusor pressure and both carbachol-induced and spontaneous detrusor strip contraction amplitude decreased gradually for the duration from 0 to 6 h, and then increased gradually to near-normal values at 24 h. Treatment of muscle strips with the p38MAK inhibitor, SB203580, inhibited carbachol-induced contractions. Smooth muscle ultrastructure damage was the highest at 6 h after AUR, and then lessened gradually during next 7 days, and ultrastructure was close to normal. Expressions of Hsp27 mRNA and protein and the proteins of the three phosphorylated forms were higher at 0 h, decreased to lower levels up to 6 h, and then gradually increased. Therefore, we conclude that rat bladder contractile function after AUR worsens during 0–6 h, and then gradually recovers. The findings of the current study suggest that Hsp27 modulates bladder smooth muscle contraction after AUR, and that phosphorylation of Hsp27 may be an important pathway modulating actin cytoskeleton dynamics in bladder smooth muscle contraction and reconstruction after injury.
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References
Choong S, Emberton M (2000) Acute urinary retention. BJU Int 85:186–201
Saito M, Miyagawa I (2001) Bladder dysfunction after acute urinary retention in rats. J Urol 165:1745–1747
Hong SK, Son H, Kim SW, Oh SJ, Choi H (2005) Effect of glycine on recovery of bladder smooth muscle contractility after acute urinary retention in rats. BJU Int 96:1403–1408
de Souza GM, Costa WS, Bruschini H, Sampaio FJ (2004) Morphological analysis of the acute effects of overdistension on the extracellular matrix of the rat urinary bladder wall. Ann Anat 186:55–59
Salinthone S, Tyagi M, Gerthoffer WT (2008) Small heat shock proteins in smooth muscle. Pharmacol Ther 119:44–54. doi:10.1016/j.pharmthera.2008.04.005
Stuart K, Thomas P (2011) Structure–functions of HspB1 (Hsp27). In: Andre-Patrick A (ed) Methods in molecular biology. Humana Press, London, pp 105–119
Efthymiou CA, Mocanu MM, de Belleroche J, Wells DJ, Latchmann DS, Yellon DM (2004) Heat shock protein 27 protects the heart against myocardial infarction. Basic Res Cardiol 99:392–394
Kim M, Park SW, Kim M, Chen SW, Gerthoffer WT, D’’gati VD, Lee HT (2010) Selective renal overexpression of human heat shock protein 27 reduces renal ischemia–reperfusion injury in mice. Am J Physiol Renal Physiol 299:F347–F358. doi:10.1152/ajprenal.00194.2010
MacIntyre DA, Tyson EK, Read M, Smith R, Yeo G, Kwek K, Chan EC (2008) Contraction in human myometrium is associated with changes in small heat shock proteins. Endocrinology 149:245–252
Park HK, Park EC, Bae SW, Park MY, Kim SW, Yoo HS, Tudev M, Ko YH, Choi YH, Kim S, Kim DI, Kim YW, Lee BB, Yoon JB, Park JE (2006) Expression of heat shock protein 27 in human atherosclerotic plaques and increased plasma level of heat shock protein 27 in patients with acute coronary syndrome. Circulation 114:886–893
Robinson AA, Dunn MJ, McCormack A, dos Remedios C, Rose ML (2010) Protective effect of phosphorylated Hsp27 in coronary arteries through actin stabilization. J Mol Cell Cardiol 49:370–379
White BG, MacPhee DJ (2011) Distension of the uterus induces HspB1 expression in rat uterine smooth muscle. Am J Physiol Regul Integr Comp Physiol 301:R1418–R1426
White BG, Williams SJ, Highmore K, Macphee DJ (2005) Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour. Reproduction 129:115–126
Kim H, Moon C, Ahn M, Byun J, Lee Y, Kim MD, Matsumoto Y, Koh CS, Shin T (2009) Heat shock protein 27 upregulation and phosphorylation in rat experimental autoimmune encephalomyelitis. Brain Res 1304:155–163
Shimizu S, Saito M, Kinoshita Y, Kazuyama E, Tamamura M, Satoh I, Satoh K (2009) Acute urinary retention and subsequent catheterization cause lipid peroxidation and oxidative DNA damage in the bladder: preventive effect of edaravone, a free-radical scavenger. BJU Int 104:713–717
Chaudhuri S, Smith PG (2008) Cyclic strain-induced HSP27 phosphorylation modulates actin filaments in airway smooth muscle cells. Am J Respir Cell Mol Biol 39:270–278
Butt E, Immler D, Meyer HE, Kotlyarov A, Laass K, Gaestel M (2001) Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets: phosphorylation-induced actin polymerization caused by HSP27 mutants. J Biol Chem 276:7108–7113
Lavoie JN, Gingras-Breton G, Tanguay RM, Landry J (1993) Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization. J Biol Chem 268:3420–3429
Graceffa P (2011) Hsp27-actin interaction. Biochem Res Int 2011:901572. doi:10.1155/2011/901572
Benndorf R, Hayess K, Ryazantsev S, Wieske M, Behlke J, Lutsch G (1994) Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity. J Biol Chem 269:20780–20784
Pichon S, Bryckaert M, Berrou E (2004) Control of actin dynamics by p38 MAP kinase—Hsp27 distribution in the lamellipodium of smooth muscle cells. J Cell Sci 117(Pt 12):2569–2577
Mounier N, Arrigo AP (2002) Actin cytoskeleton and small heat shock proteins: how do they interact? Cell Stress Chaperones 7:167–176
Brophy CM, Woodrum D, Dickinson M, Beall A (1998) Thrombin activates MAPKAP2 kinase in vascular smooth muscle. J Vasc Surg 27:963–969
Larsen JK, Yamboliev IA, Weber LA, Gerthoffer WT (1997) Phosphorylation of the 27-kDa heat shock protein via p38 MAP kinase and MAPKAP kinase in smooth muscle. Am J Physiol 273(5 Pt 1):L930–L940
Bitar KN, Hillemeier C, Biancani P, Balazovich KJ (1991) Regulation of smooth muscle contraction in rabbit internal anal sphincter by protein kinase C and Ins(1,4,5)P3. Am J Physiol 260(4 Pt 1):G537–G542
Quinlan R (2002) Cytoskeletal competence requires protein chaperones. Prog Mol Subcell Biol 28:219–233
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The research described in this article was funded by the National Natural Sciences Fund (No. 81000288, 81270845) and aided by Special Foundation for Young Scientists of Third Military Medical University (No. 2010XQN23).
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Zhiyong Xiong and Yongquan Wang have contributed equally, Zhansong Zhou and Gensheng Lu have contributed equally.
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Xiong, Z., Wang, Y., Gong, W. et al. Expression of Hsp27 correlated with rat detrusor contraction after acute urinary retention. Mol Cell Biochem 381, 257–265 (2013). https://doi.org/10.1007/s11010-013-1709-4
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DOI: https://doi.org/10.1007/s11010-013-1709-4