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Baicalein protects mice against radiation-induced DNA damages and genotoxicity

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Abstract

Baicalein is the major flavonoid extracted from the root of Scutellaria baicaleins. This flavonoid is used extensively in Chinese herbal medicine. In the present study baicalein is evaluated for its radioprotective properties. Human blood cells when exposed to the γ-radiation ex vivo in presence of baicalein underwent the reduced DNA damage compared to the control. Baicalein administration prior to the whole-body γ-radiation (4 Gy) exposure of mice resulted in protecting the damage to the DNA as measured in their blood cells by alkaline comet assay. Mice when exposed to the radiation (whole body; 1.7 Gy) resulted in damage to the bone marrow as measured by micronucleated reticulocyte (MNRET) formation. Baicalein pre-treatment reduces the radiation induced damage to the bone marrow cells, as there was decrease in the percentage MNRET formation. These findings indicate radio-protecting ability of baicalein.

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References

  1. Thielen H (2012) The Fukushima Daiichi nuclear accident: an overview. Health Phys 103:169–174

    Article  PubMed  CAS  Google Scholar 

  2. Nair CKK, Parida DK, Nomura T (2001) Radioprotectors in radiotherapy. J Radiat Res 42:21–37

    Article  PubMed  CAS  Google Scholar 

  3. Greenberger JS (2009) Radioprotection. In Vivo 23:323–336

    PubMed  CAS  Google Scholar 

  4. Pellmar TC, Rockwell S (2005) Radiological/nuclear threat countermeasures working group. Priority list of research areas for radiological nuclear threat countermeasures. Radiat Res 163:115–123

    Article  PubMed  CAS  Google Scholar 

  5. Weiss JF, Landauer MR (2003) Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology 189:1–20

    Article  PubMed  CAS  Google Scholar 

  6. Gao D, Sakurai K, Katoh M, Chen J, Ogiso T (1996) Inhibition of microsomal lipid peroxidation by baicalein: a possible formation of an iron–baicalein complex. Biochem Mol Biol Int 39:215–255

    PubMed  CAS  Google Scholar 

  7. Tilak JC, Devasagayam TPA (2003) Cellular membrane protection against reactive oxygen species by Terminalia arjuna and its active component Baicalein. J Clin Biochem Nutr 39:75–87

    Article  Google Scholar 

  8. Ghosh D, Pal S, Saha C, Chakrabarti AK, Datta SC, Dey SK (2012) Black tea extract: a supplementary antioxidant in radiation-induced damage to DNA and normal lymphocytes. J Environ Pathol Toxicol Oncol 31:155–166

    Article  PubMed  CAS  Google Scholar 

  9. Arora R, Chawla R, Dhaker AS, Adhikari M, Sharma J, Singh S, Gupta D, Kumar R, Sharma A, Sharma RK, Tripathi RP (2010) Podophyllum hexandrum as a potential botanical supplement for the medical management of nuclear and radiological emergencies (NREs) and free radical-mediated ailments: leads from in vitro/in vivo radioprotective efficacy evaluation. J Diet Suppl 7:31–50

    Article  PubMed  Google Scholar 

  10. Min LW (2009) New therapeutic aspects of flavones. The anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev 35:57–68

    Article  Google Scholar 

  11. Li C, Lin G, Zuo Z (2011) Pharmacological effects and pharmacokinetics properties of Radix Scutellariae and its bioactive flavones. Biopharm Drug Disp 32:427–445

    Article  Google Scholar 

  12. Singh NP (2000) Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res 455:111–127

    Article  PubMed  CAS  Google Scholar 

  13. Gandhi NM, Nair CKK (2005) Radiation protection by Terminalia chebula: some mechanistic aspects. Mol Cell Biochem 277:43–48

    Article  PubMed  CAS  Google Scholar 

  14. Konca K, Larkoff A, Banasik A, Lisowska H, Kuszewski T, Gozdz S et al (2000) A cross platform public domain PC image analysis program for the comet assay. Mutat Res 534:15–20

    Google Scholar 

  15. Hayashi M, Morita T, Kodama Y, Sofuni T, Ishidate M Jr (1990) The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides. Mutat Res 245:245–249

    Article  PubMed  CAS  Google Scholar 

  16. Belli M, Sapora O, Tabocchini MA (2000) Molecular targets in cellular response to ionizing radiation and implications in space radiation protection. J Radiat Res 43(Suppl):S13–S19

    Google Scholar 

  17. Hofer M, Mazur L, Pospicil M, Weiterova L, Znojil V (2000) Radiprotective action of extracellular adenosine on bone marrow cells in mice exposed to gamma rays as assayed by micronucleus test. Radiat Res 154:217–221

    Article  PubMed  CAS  Google Scholar 

  18. Gao Z, Huang K, Yang X, Xu H (1999) Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta 1472:643–650

    Article  PubMed  CAS  Google Scholar 

  19. Adhikari S, Tilak JC, Devasagayam TP (2011) Free radical reactions of a naturally occurring flavones baicalein and possible mechanisms towards its membrane protective properties. Ind J Biochem Biophys 48:275–282

    CAS  Google Scholar 

  20. Baowan L (2009) Integrating comprehensive and alternative medicine into stroke. In: Ross Ronald (ed) Complementary and alternative therapies in the ageing population. An evidence based approach. Elsevier, New York, pp 260–261

    Google Scholar 

  21. Lai MY, Hsiu SL, Chen CC, Hou YC, Chao PD (2003) Urinary pharmacokinetics of baicalein, wogonin and their glycosides after oral administration of Scutellariae Radix in humans. Biol Pharm Bull 26:79–83

    Article  PubMed  CAS  Google Scholar 

  22. Kim DH, Jang S, Lee HK, Jung EA, Lee KY (1996) Metabolism of glycyrrhizin and baicalin by human intestinal bacteria. Arch Pharmacol Res 19:292–296

    Article  CAS  Google Scholar 

  23. Regan-Shaw S, Nihal M, Nihal A (2007) Dose translation from animal to human studies revisited. Faseb J 22:659–661

    Article  Google Scholar 

  24. Brenner DJ, Elliston CD (2004) Estimation of radiation risks potentially associated with full-body screening. Radiology 232:735–738

    Article  PubMed  Google Scholar 

  25. Pearce MS, Salotti JA, Little M, McHugh K, Lee C, Kim KP et al (2012) Radiation exposure from CT scans in childhood and subsequent risk of leukemia and brain tumors, a retrospective cohort study. Lancet 380(9840):499–505

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was supported by Department of Atomic Energy, Government of India. The author would like to acknowledge the help of Ms. Anjali C.H. and Mr. Melvin Mathew and technical assistance of Mr. P. M. Gonsalves.

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  1. Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, 400085, Mumbai, India

    Nitin Motilal Gandhi

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  1. Nitin Motilal Gandhi
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Correspondence to Nitin Motilal Gandhi.

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Gandhi, N.M. Baicalein protects mice against radiation-induced DNA damages and genotoxicity. Mol Cell Biochem 379, 277–281 (2013). https://doi.org/10.1007/s11010-013-1649-z

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  • DOI: https://doi.org/10.1007/s11010-013-1649-z

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