Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein
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This study aims to determine the differentially expressed proteins in the pancreatic acinar cells undergoing apoptosis and oncosis stimulated with caerulein to explore different cell death process of the acinar cell. AR42J cells were treated with caerulein to induce cell model of acute pancreatitis. Cells that were undergoing apoptosis and oncosis were separated by flow cytometry. Then differentially expressed proteins in the two groups of separated cells were detected by shotgun liquid chromatography-tandem mass spectrometry. The results showed that 11 proteins were detected in both apoptosis group and oncosis group, 17 proteins were detected only in apoptosis group and 29 proteins were detected only in oncosis group. KEGG analysis showed that proteins detected only in apoptosis group were significantly enriched in 10 pathways, including ECM-receptor interaction, cell adhesion molecules, and proteins detected only in oncosis group were significantly enriched in three pathways, including endocytosis, base excision repair, and RNA degradation. These proteins we detected are helpful for us to understand the process of cell death in acute pancreatitis and may be useful for changing the death mode of pancreatic acinar cells, thus attenuating the severity of pancreatitis.
KeywordsProteomics Acute pancreatitis Apoptosis Oncosis
This work was supported by the National Natural Science Foundation of China (30972907, 81070373); the National Science Foundation for Post-doctoral Scientists of China (Grant Number: 20090451020); Heilongjiang Postdoctoral Grant (Grant Number: LRB08-503); Science Foundation of the First Affiliated Hospital of Harbin Medical University (Grant Number: B08-003). Authors are most grateful to Prof. Wei Jiang and Dr. Wei Li (College of Bioinformatics Science and Technology, Harbin Medical University) for their help with sample bioinformatics analysis. We thank RCPA (Research Centre for Proteome Analysis, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China) for providing the proteomics technology.
Conflict of interest
The authors declare that they have no competing interests.
- 5.Oiva J, Mustonen H, Kylänpää ML, Kyhälä L, Alanärä T, Aittomäki S, Siitonen S, Kemppainen E, Puolakkainen P, Repo H (2010) Patients with acute pancreatitis complicated by organ failure show highly aberrant monocyte signaling profiles assessed by phospho-specific flow cytometry. Crit Care Med 38:1702–1708CrossRefPubMedGoogle Scholar
- 7.Xue DB, Zhang WH, Yun XG, Song C, Zheng B, Shi XY, Wang HY (2007) Regulating effects of arsenic trioxide on cell death pathways and inflammatory reactions of pancreatic acinar cells in rats. Chin Med J (Engl) 120:690–695Google Scholar
- 16.Löw P, Bussell K, Dawson SP, Billett MA, Mayer RJ, Reynolds SE (1997) Expression of a 26s proteasome ATPase subunit, MS73, in muscles that undergo developmentally programmed cell death, and its control by ecdysteroid hormones in the insect Manduca sexta. FEBS Lett 400:345–349CrossRefPubMedGoogle Scholar
- 24.Ghayur T, Banerjee S, Hugunin M, Butler D, Herzog L, Carter A, Quintal L, Sekut L, Talanian R, Paskind M, Wong W, Kamen R, Tracey D, Allen H (2000) An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J Biol Chem 275:27823–27831Google Scholar