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AdipoR1 and AdipoR2 gene expression are regulated by thyroid hormones in adipose tissue

Molecular and Cellular Biochemistry volume 377, pages 55–63 (2013)Cite this article

Abstract

The aim of this study was to examine whether the relative gene expression of AdipoR1 and AdipoR2 in rat adipose tissue is altered by thyroid hormones, and whether this might relate to their circulating thyroid hormones and adiponectin levels. Hyper- and hypothyroidism were induced by daily oral administration of levothyroxine and methimazole in rats, respectively, over a 42 days period. Real-time PCR analysis was performed to evaluate the changes in AdipoR1 and AdipoR2 mRNA levels in the adipose tissue on days 15, 28, 42, and also 2 weeks after the cessation of treatment. In response to treatment with methimazole, mRNA levels of AdipoR1 and AdipoR2 decreased in the white adipose tissue compared to the euthyroid rats (p < 0.05). This decline was reversible 2 weeks after treatment cessation. The mRNA levels of AdipoR1 and AdipoR2 were increased in the hyperthyroid group of animals compared to euthyroid control (p < 0.05), and its changes were reversible 2 weeks after treatment cessation (P < 0.05). Adiponectin receptors gene expression levels in the adipose tissue of treated animals have positive correlations with thyroid hormones concentrations. Our results suggest that AdipoR1 and AdipoR2 gene expression is regulated by thyroid hormones in hypo- and hyperthyroidism.

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Acknowledgments

This study was financially supported by the Shahid Chamran University of Ahvaz (Grant No. 89/3/02/44305) and Shiraz University.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Samira Seifi & Mohammad Reza Tabandeh

  2. Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Saeed Nazifi

  3. Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Mehdi Saeb

Authors
  1. Samira Seifi
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  2. Saeed Nazifi
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  3. Mohammad Reza Tabandeh
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  4. Mehdi Saeb
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Correspondence to Mohammad Reza Tabandeh.

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Seifi, S., Nazifi, S., Tabandeh, M.R. et al. AdipoR1 and AdipoR2 gene expression are regulated by thyroid hormones in adipose tissue. Mol Cell Biochem 377, 55–63 (2013). https://doi.org/10.1007/s11010-013-1570-5

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  • DOI: https://doi.org/10.1007/s11010-013-1570-5

Keywords

  • Adiponectin receptors
  • Gene expression
  • Adipose tissue
  • Hypothyroidism
  • Hyperthyroidism