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Investigation of in vitro and in vivo antioxidant potential of secoisolariciresinol diglucoside

Molecular and Cellular Biochemistry volume 373, pages 179–187 (2013)Cite this article

Abstract

The present study was designed to evaluate the in vitro and in vivo ameliorative antioxidant potential of secoisolariciresinol diglucoside (SDG). In vitro antioxidant activity of synthetic SDG was carried out using DPPH, reducing power potency, and DNA protection assays. Wistar albino rats weighing 180–220 g were used for in vivo studies and liver damage was induced in the experimental animals by a single intraperitoneal (I.P.) injection of CCl4 (2 g/kg b.w.). Intoxicated animals were treated orally with synthetic SDG at (12.5 and 25 mg/kg b.w.) and Silymarin (25 mg/kg) for 14 consecutive days. The levels of catalase (CAT), superoxide dismutase (SOD), peroxidase (POX), and lipid peroxidase (LPO) were measured in liver and kidney homogenates. The synthetic SDG exerts high in vitro antioxidant potency as it could scavenge DPPH at a IC50 value of 78.9 μg/ml and has dose-dependent reducing power potency and protected DNA at 0.5 mg/ml concentration. Oral administration of synthetic SDG at 12.5 and 25 mg/kg b.w. showed significant protection compared to Silymarin (25 mg/kg) and the activities of CAT, SOD, and POX were markedly increased (P < 0.05), whereas LPO significantly decreased (P < 0.001) in a dose-dependent manner in liver and kidney in both pre- and post-treatment groups when compared to toxin-treated group. The results of in vitro and in vivo investigations revealed that synthetic SDG at 25 mg/kg b.w. is associated with beneficial changes in hepatic enzyme activities and thereby plays a key role in the prevention of oxidative damage in immunologic system.

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References

  1. Zheng RL, Huang ZY (2001) Reactive oxygen species. In: Zheng RL, Huang ZY (eds) Free radical in medical and agricultural science. China Higher Education Press and Springer Press, Beijing, pp 17–27

    Google Scholar 

  2. Ames BN, Shigenaga MK, Hagen TM (1993) Oxidants, antioxidants and the degenerative diseases of aging. Proc Natl Acad Sci 90:7915–7922

    PubMed  Article  CAS  Google Scholar 

  3. Halliwell B, Gutteridge JM (1989) Free radicals in biology and medicine. Clarendon Press, Oxford

    Google Scholar 

  4. Seifried HE, Anderson DE, Fisher EI, Milner JA (2007) A review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 18:567–579

    PubMed  Article  CAS  Google Scholar 

  5. Wickens AP (2001) Ageing and the free radical theory. Respir Physiol 128:379–391

    PubMed  Article  CAS  Google Scholar 

  6. Aruoma OI (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chem 32:671–683

    Article  CAS  Google Scholar 

  7. Peterszegi G, Robert AM, Robert L (2003) Protection by l-fucose and fucose-rich polysaccharides against ROS-produced cell death in presence of ascorbate. Biomed Pharmacother 57:130–133

    PubMed  Article  CAS  Google Scholar 

  8. Murthy KC, Jayaprakasha GK, Singh RP (2002) Studies on antioxidant activity of pomegranate (Punica granatum) peel extracts using in vivo models. J Agric Food Chem 50:4791–4795

    Article  CAS  Google Scholar 

  9. Rajesha J, Kotamballi N, Chidambara M, Karun KM, Basavaraj M, Gokare AR (2006) Antioxidant potentials of flaxseed by in vivo model. J Agric Food Chem 54:3794–3799

    PubMed  Article  CAS  Google Scholar 

  10. Prasad K (1997) Hydroxyl radical-scavenging property of secoisolariciresinol diglucoside (SDG) isolated from flaxseed. Mol Cell Biochem 168:117–123

    PubMed  Article  CAS  Google Scholar 

  11. Prasad K, Kalra J (1993) Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E. Am Heart J 125:958–973

    PubMed  Article  CAS  Google Scholar 

  12. Prasad K (1998) Prevention of IDDM in BBdp rats by secoisolariciresinol diglucoside (SDG) isolated from flaxseed. Diabetes 47(I): A360

    Google Scholar 

  13. Jenab M, Thompson LU (1996) The influence of flaxseed and lignans on colon carcinogenesis and b-glucuronidase activity. Carcinogenesis 17:1343–1348

    PubMed  Article  CAS  Google Scholar 

  14. Serraino M, Thompson LU (1991) The effect of flaxseed supplementation and early markers of mammary carcinogenesis. Cancer Lett 60:135–142

    PubMed  Article  CAS  Google Scholar 

  15. Kitts DD, Thompson LU, Yuan YV, Wijewickreme AN (1999) Antioxidant activity of the flaxseed lignin secoisolariciresinol diglycoside and its mammalian lignan metabolites enterodiol and enterolactone. Mol Cell Biochem 202:91–100

    PubMed  Article  CAS  Google Scholar 

  16. Lai LS, Chou ST, Chao WW (2001) Studies on the antioxidative activities of Hsiantsao (Mesonaprocumbens Hemsl) leaf gum. J Agric Food Chem 49:963–968

    PubMed  Article  CAS  Google Scholar 

  17. Yen GC, Chen HY (1995) Antioxidant activity of various tea extracts in relation to their ant mutagenicity. J Agric Food Chem 43:27–32

    Article  CAS  Google Scholar 

  18. Lee J, Kim J, Jang Y (2002) Antioxidant property of an ethanol extract of the stem of Opuntiaficus-indicavar. Saboten. J Agric Food Chem 50:6490–6496

    PubMed  Article  CAS  Google Scholar 

  19. Aebi H et al (1984) Catalyse in vitro methods of analysis. Methods Enzymol 105:121–125

    PubMed  Article  CAS  Google Scholar 

  20. Beuchamp, Fedovich BC (1976) Superoxide dismutase; improved assay and an assay applicable to acrylamide gel. Anal Biochem 10:276–287

    Google Scholar 

  21. Alexander NM (1962) A spectrophotometric assay for iodide oxidation by thyroid peroxidase. Anal Biochem 4:341–345

    PubMed  Article  CAS  Google Scholar 

  22. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310

    PubMed  Article  CAS  Google Scholar 

  23. Lowry OH, Rosenberg NJ, Faw AL, Randall RJ (1951) Protein measurement with Folin reagent. J Biol Chem 193:265–267

    PubMed  CAS  Google Scholar 

  24. Recknagel RO (1967) Carbon tetrachloride hepatotoxicity. Pharmacol Rev 19:145–208

    PubMed  CAS  Google Scholar 

  25. Gil MI, Tomas-Barberan FA, Hesspierce B, Holecroft DM, Kader AA (2000) Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem 48:4581–4589

    PubMed  Article  CAS  Google Scholar 

  26. Masella R, Cantafora A, Modesti D, Cardilli A, Gennaro L, Bocca A, Coni E (1999) Antioxidant activity of 3,4-DHPEAEA and protocatechuic acid: a comparative assessment with other olive oil biphenols. Redox Rep 4:113–121

    PubMed  Article  CAS  Google Scholar 

  27. Sies H, Groot H (1992) Role of reactive oxygen species in cell toxicity. Toxicol Lett 64:547–551

    PubMed  Article  Google Scholar 

  28. Schraufstatter IU, Hyslop PA, Jackson J, Cochrane CG (1995) Oxidant induced DNA damage of target cells. J Clin Invest 82:1040–1050

    Article  Google Scholar 

  29. Buttke TM, Sandstrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10

    PubMed  Article  CAS  Google Scholar 

  30. Hosseinian FS, Muir AD, Westcott ND, Krol ES (2007) AAPH-mediated antioxidant reactions of secoisolariciresinol and SDG. Org Biomol Chem 5:644–654

    PubMed  Article  CAS  Google Scholar 

  31. Jayaprakasha GK, Chidambara Murthy KN, Bhimanagouda SP (2008) Antioxidant activities of polyphenol containing extracts from citrus. Funct Food Health 24:264–276

    Article  Google Scholar 

  32. Ahmed E, Moneim Abdel (2011) Antioxidant activities of Punica granatum (pomegranate) peel extract on brain of rats. J Med Plants Res 6(2):195–199

    Google Scholar 

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Acknowledgments

The authors are grateful for the financial assistance provided by the Department of Science & Technology, New Delhi, to carry out this research work. We are thankful to the Departments of Studies in Chemistry/Zoology, University of Mysore, Mysore, for providing facilities and animals; and Indian Institute of Science, Bangalore for the assistance with structural analysis.

Conflict of interest

The authors have declared no conflict of interest.

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  1. Department of Biochemistry, Yuvaraja’s College, University of Mysore, Mysore, 570005, Karnataka, India

    Sadiq S. Moree & J. Rajesha

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  1. Sadiq S. Moree
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  2. J. Rajesha
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Correspondence to J. Rajesha.

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Moree, S.S., Rajesha, J. Investigation of in vitro and in vivo antioxidant potential of secoisolariciresinol diglucoside. Mol Cell Biochem 373, 179–187 (2013). https://doi.org/10.1007/s11010-012-1487-4

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  • DOI: https://doi.org/10.1007/s11010-012-1487-4

Keywords

  • Synthetic SDG
  • Antioxidant activity
  • DNA protection
  • Silymarin
  • Hepatic enzymes