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Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of ∙NO

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Abstract

In this study, the effects of l-arginine-nitric-oxide (∙NO)-producing pathway on protein content of ubiquitin, as an important component of ubiquitin–proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide (∙NO), besides well established roles, is involved in this aspect of structure remodeling, as well.

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Acknowledgments

This study was supported by the Ministry of Education and Science of the Republic of Serbia, Grant No. 173055. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Department of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade, Serbia

    Milica Vucetic, Vesna Otasevic, Ana Stancic, Aleksandra Jankovic, Biljana Buzadzic & Bato Korac

  2. Faculty of Biology, Institute of Zoology and Center for Electron Microscopy, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia

    Milica Markelic, Igor Golic, Ksenija Velickovic & Aleksandra Korac

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  1. Milica Vucetic
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  2. Vesna Otasevic
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  3. Ana Stancic
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  4. Aleksandra Jankovic
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  5. Milica Markelic
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  6. Igor Golic
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  7. Ksenija Velickovic
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  8. Biljana Buzadzic
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  9. Aleksandra Korac
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  10. Bato Korac
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Correspondence to Bato Korac.

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Vucetic, M., Otasevic, V., Stancic, A. et al. Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of ∙NO. Mol Cell Biochem 368, 189–193 (2012). https://doi.org/10.1007/s11010-012-1359-y

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  • DOI: https://doi.org/10.1007/s11010-012-1359-y

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