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Regulation of the transcriptional activation of CTRP3 in chondrocytes by c-Jun

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Abstract

Although C1qTNF-related protein 3 (CTRP3) is highly expressed in cartilage and is involved in the proliferation and migration of chondrocytes, the molecular mechanism governing the transcriptional regulation of CTRP3 in chondrocytes is largely unknown. Here, we demonstrated the mechanism of transcriptional activation of the CTRP3 gene in chondrogenic cells. We observed that c-Jun stimulated CTRP3 promoter activity in ATDC5 cells. Deletion analysis of the CTRP3 promoter showed that the c-Jun-responsive region is localized at the position between −359 and −176. Mutation of the AP-1 binding site in this region eliminated c-Jun-mediated CTRP3 promoter activity. A promoter enzyme immunoassay and a chromatin immunoprecipitation assay showed that c-Jun binds directly to the AP-1 binding site at the region −184/−177 of the mouse CTRP3 promoter. Thus, these results demonstrated that c-Jun is a cis-acting element for CTRP3 regulation in chondrogenic cells.

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Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0021626, 2011-0016102); by a grant from 21C Frontier Functional Proteomics Project from Ministry of Education, Science and Technology (M108KM010008-08K1301-00810); and by the grant No. RTI04-01-01 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE).

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Dongguk University, 707 Sukjang-dong, Gyeongju, 780-714, Republic of Korea

    Mi-Jin Kim, Eun-Ju Park, Wan Lee & Seung-Yoon Park

  2. Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, 700-422, Republic of Korea

    Jung-Eun Kim

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  1. Mi-Jin Kim
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  2. Eun-Ju Park
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Correspondence to Seung-Yoon Park.

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Mi-Jin Kim, Eun-Ju Park, and Wan Lee contributed equally to this work.

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Kim, MJ., Park, EJ., Lee, W. et al. Regulation of the transcriptional activation of CTRP3 in chondrocytes by c-Jun. Mol Cell Biochem 368, 111–117 (2012). https://doi.org/10.1007/s11010-012-1349-0

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  • DOI: https://doi.org/10.1007/s11010-012-1349-0

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