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Gender-specific effects of exercise on cardiac pathology in Na+/H+ exchanger overexpressing mice

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Abstract

The Na+/H+ exchanger isoform 1 (NHE1) has been implicated in various cardiac pathologies including ischemia/reperfusion damage to the myocardium and cardiac hypertrophy. It is known that NHE1 levels increase in cardiac disease and we have recently demonstrated that expression of an activated NHE1 protein promotes cardiac hypertrophy in the mouse myocardium. We examined the gender-specific effects of exercise in combination with elevated cardiac expression of NHE1 on the myocardium in mice. Control mice and transgenic mice expressing elevated levels of wild type NHE1 and activated NHE1 were examined. There were gender-specific differences in the effects of NHE1 with exercise. Exercised wild type male mice showed a tendency toward increased heart weight. This was not apparent in female mice expressing elevated NHE1 levels. In some transgenic female mice, there was a significant decrease in the size of the exercised hearts, which was different from what occurred with male mice. Body weight was maintained in exercised control and transgenic male mice; however, it decreased in female mice with exercise more so in transgenic female mice expressing elevated levels of NHE1. Female mice expressing activated NHE1 had elevated HW/BW ratios compared to males, and this was exaggerated by exercise. These results suggest that gender-specific activation of NHE1 may be critical and that NHE1 plays a more critical role in promoting some types of hypertrophy in females in comparison with males.

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Acknowledgments

This study was supported by funding from the Canadian Institutes of Health Research to LF. LF is supported by an Alberta Ingenuity for Health Research Scientist award.

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Authors and Affiliations

  1. Department of Biochemistry, University Alberta, Edmonton, AB, T6G 2H7, Canada

    Heather Vandertol Vanier, Xiuju Li & Larry Fliegel

  2. College of Pharmacy, Qatar University, Doha, Qatar

    Fatima Mraiche

Authors
  1. Heather Vandertol Vanier
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  2. Fatima Mraiche
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  3. Xiuju Li
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  4. Larry Fliegel
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Correspondence to Larry Fliegel.

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Vanier, H.V., Mraiche, F., Li, X. et al. Gender-specific effects of exercise on cardiac pathology in Na+/H+ exchanger overexpressing mice. Mol Cell Biochem 368, 103–110 (2012). https://doi.org/10.1007/s11010-012-1348-1

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  • DOI: https://doi.org/10.1007/s11010-012-1348-1

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