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Fhl1 as a downstream target of Wnt signaling to promote myogenesis of C2C12 cells

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Abstract

Previous studies have shown that Wnt signaling is involved in postnatal mammalian myogenesis; however, the downstream mechanism of Wnt signaling is not fully understood. This study reports that the murine four-and-a-half LIM domain 1 (Fhl1) could be stimulated by β-catenin or LiCl treatment to induce myogenesis. In contrast, knockdown of the Fhl1 gene expression in C2C12 cells led to reduced myotube formation. We also adopted reporter assays to demonstrate that either β-catenin or LiCl significantly activated the Fhl1 promoter, which contains four putative consensus TCF/LEF binding sites. Mutations of two of these sites caused a significant decrease in promoter activity by luciferase reporter assay. Thus, we suggest that Wnt signaling induces muscle cell differentiation, at least partly, through Fhl1 activation.

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Abbreviations

Fhl1:

Four-and-a-half LIM domain 1

LiCl:

Lithium chloride

TCF:

T-cell specific factor

LEF:

Lymphoid enhancer-binding factor

PCR:

Polymerase chain reaction

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Acknowledgments

We thank Dr. Pin Ling for supplying the pcDNA plasmid and Dr. Hsi-Tien Wu for the C2C12. This study was supported by the NSC (National Science Council, Taiwan) under grant numbers 97-2311-B-415-002- and 98-2311-B-415-001-MY3.

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  1. Department of Bioagricultural Sciences, National Chiayi University, No. 300 Syuefu Rd., Chiayi, 60004, Taiwan, ROC

    Jing-Yu Lee, I-Chun Chien, Win-Yu Lin, Shao-min Wu, Bo-Huei Wei, Yu-En Lee & Hu-Hui Lee

  2. Ph.D Program of Agriculture Science, National Chiayi University, Chiayi, Taiwan, ROC

    Jing-Yu Lee

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  1. Jing-Yu Lee
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Lee, JY., Chien, IC., Lin, WY. et al. Fhl1 as a downstream target of Wnt signaling to promote myogenesis of C2C12 cells. Mol Cell Biochem 365, 251–262 (2012). https://doi.org/10.1007/s11010-012-1266-2

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