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Macrophages actively accumulate malonyldialdehyde-modified but not enzymatically oxidized low density lipoprotein

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Abstract

In this study, we show that low density lipoproteins (LDL) from human blood plasma which was oxidized by animal C-15 lipoxygenase is taken up by cultivated human macrophages with the same effectiveness as with non-oxidized (native) LDL. At the same time malonyldialdehyde-modified LDL is captured by cultivated macrophages very actively. Based on differences in catabolism of LDL with various levels of primary and secondary products of free-radical oxidation, it was offered to discriminate between the oxidized LDL itself (lipohydroperoxide-rich LDL) and the LDL that was chemically modified by free-radical oxidation secondary products of aldehyde nature. In this respect, aldehyde-modified but not oxidized (lipohydroperoxide-containing) LDL is atherogenic.

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Abbreviations

LDL:

Low density lipoprotein

LOX:

C-15 Lipoxygenase

MDA:

Malonyldialdehyde

PBS:

Phosphate-buffered saline

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Authors and Affiliations

  1. Russian Cardiology Research Complex, 3rd Cherepkovskaya street, 15a, Moscow, Russia, 121552

    V. Z. Lankin, A. K. Tikhaze & E. M. Kumskova

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  1. V. Z. Lankin
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  2. A. K. Tikhaze
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  3. E. M. Kumskova
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Correspondence to V. Z. Lankin.

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Lankin, V.Z., Tikhaze, A.K. & Kumskova, E.M. Macrophages actively accumulate malonyldialdehyde-modified but not enzymatically oxidized low density lipoprotein. Mol Cell Biochem 365, 93–98 (2012). https://doi.org/10.1007/s11010-012-1247-5

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