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Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells

Molecular and Cellular Biochemistry volume 363, pages 437–444 (2012)Cite this article

Abstract

Syndecan-4 (S4) is a cell membrane heparan sulfate proteoglycan that plays a role in satellite cell mediated myogenesis. S4 modulates the proliferation of myogenic satellite cells, but the mechanism of how S4 functions during myogenesis is not well understood. In other cell systems, S4 has been shown to form oligomers in the cell membrane and interact through its cytoplasmic domain with the cytoskeletal protein α-actinin. This study addressed if S4 forms oligomers and interacts with α-actinin in muscle. The S4 cytoplasmic domain was found to interact with α-actinin in a phosphatidylinositol-4,5-bisphosphate dependent manner, but did not associate with vinculin. Through confocal microscopy, both S4 and syndecan-4 without the cytoplasmic domain were localized to the cell membrane. Although the cytoplasmic domain was necessary for the interaction with α-actinin, S4 oligomer formation occurred in the absence of the cytoplasmic domain. These data indicated that S4 function in skeletal muscle is mediated through the formation of oligomers and interaction with the cytoskeletal protein α-actinin.

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Abbreviations

BSA:

Bovine serum albumin

FGF2:

Fibroblast growth factor 2

IPTG:

Isopropyl-β-1-thiogalactopyranoside

IP:

Immunoprecipitated

PKCα:

Protein kinase C α

PVDF:

Polyvinylidene fluoride membrane

PIP2 :

Phosphatidylinositol-4,5-bisphosphate

siRNA:

Small interfering RNA

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel

S4:

Syndecan-4

S4C:

Syndecan-4 without the cytoplasmic domain

TBS-T:

Tris-buffered saline containing Tween 20

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Acknowledgments

Salary and partial research support to S.G.V. was provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, the Midwest Poultry Research Consortium to S.G.V. and D.C.M., National Institute of Food and Agriculture Multistate Project NC-1184 to S.G.V. and D.C.M, and National Research Initiative Competitive Grant no. 2009-35503-05176 to S.G.V. from the USDA National Institute of Food and Agriculture.

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Affiliations

  1. Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 213 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH, 44691, USA

    Jonghyun Shin, Yan Song & Sandra G. Velleman

  2. Department of Animal Science, South Dakota State University, Brookings, SD, 57007, USA

    Douglas C. McFarland

Authors
  1. Jonghyun Shin
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  2. Yan Song
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  3. Douglas C. McFarland
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  4. Sandra G. Velleman
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Correspondence to Sandra G. Velleman.

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Shin, J., Song, Y., McFarland, D.C. et al. Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells. Mol Cell Biochem 363, 437–444 (2012). https://doi.org/10.1007/s11010-011-1198-2

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  • DOI: https://doi.org/10.1007/s11010-011-1198-2

Keywords

  • α-Actinin
  • Muscle
  • Oligomers
  • Satellite cell
  • Syndecan-4