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Chronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes

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Abstract

Since mild hyperhomocysteinemia is a risk factor for cardiovascular and cerebral diseases and extracellular nucleotides/nucleosides, which are controlled by the enzymatic action of ectonucleotidases, can induce an immune response, in the present study, we investigated the effect of chronic mild hyperhomocysteinemia on ectonucleotidase activities and expression in lymphocytes from mesenteric lymph nodes and serum of adult rats. For the chronic chemically induced mild hyperhomocysteinemia, Hcy (0.03 μmol/g of body weight) or saline (control) were administered subcutaneously from the 30th to the 60th day of life. Results showed that homocysteine significantly decreased ATP, ADP, and AMP hydrolysis in lymphocytes of adult rats. E-NTPDases transcriptions were not affected, while the ecto-5′-nucleotidase transcription was significantly decreased in mesenteric lymph nodes of hyperhomocysteinemic rats. ATP, ADP, and AMP hydrolysis were not affected by homocysteine in rat serum. Our findings suggest that Hcy in levels similar to considered risk factor to development of vascular diseases modulates the ectonucleotidases, which could lead to a pro-inflammatory status.

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Acknowledgments

This study was supported in part by grants from the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq–Brazil).

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Authors and Affiliations

  1. Laboratório de Neuroproteção e Doenças Metabólicas, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil

    Emilene B. S. Scherer, Luiz Eduardo B. Savio, Fernanda C. Vuaden, Andréa G. K. Ferreira & Angela T. S. Wyse

  2. Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Maurício R. Bogo & Carla D. Bonan

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  1. Emilene B. S. Scherer
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  2. Luiz Eduardo B. Savio
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  3. Fernanda C. Vuaden
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  4. Andréa G. K. Ferreira
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  7. Angela T. S. Wyse
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Correspondence to Angela T. S. Wyse.

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Scherer, E.B.S., Savio, L.E.B., Vuaden, F.C. et al. Chronic mild hyperhomocysteinemia alters ectonucleotidase activities and gene expression of ecto-5′-nucleotidase/CD73 in rat lymphocytes. Mol Cell Biochem 362, 187–194 (2012). https://doi.org/10.1007/s11010-011-1141-6

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