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Cardiac dysfunction subsequent to chronic ozone exposure in rats

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Abstract

A number of advancements have been made toward identifying the risk factors associated with cardiovascular disease (CVD) and have resulted in a decline in mortality. However, many patients with cardiac disease show no established previous risk. Thus, it appears that other unknown factors contribute to the pathophysiology of CVD. Out of 350,000 sudden cardiac deaths each year in the United States, 60,000 deaths have been linked to air pollution, suggesting a detrimental role of environmental pollutants in the development of CVD. This study tested the hypothesis that chronic ozone (O3) exposure diminishes myocardial function in healthy population. Male Sprague–Dawley rats were exposed 8 h/day for 28 and 56 days to filtered air or 0.8 ppm O3. In vivo cardiac function was assessed by measuring LVDP, +dP/dt, −dP/dt, and LVEDP 24 h after termination of the O3 exposure. Compared to rats exposed to filtered air, LVDP, +dP/dt, and −dP/dt were significantly decreased, and LVEDP was significantly increased in O3 exposed animals. This attenuation of cardiac function was associated with increased myocardial TNF-alpha levels and lipid peroxidation as well as decreased myocardial activities of superoxidase dismutase and interleukin-10 levels. These novel findings suggest myocardial dysfunction subsequent to chronic O3 exposure in normal adult rats may be associated with a decrease in antioxidant reserve and with an increased production of inflammatory mediators.

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Acknowledgments

Dr. Rama Surya Prakash Perepu was a graduate student with TAMUK. This study was made possible by the United States Environmental Protection Agency Grant (USEPA Grant # IT-83404401-0) and Texas A&M Health Science Research Development Grant (Act# 134403-35402) and funds from TAMHSC Research Startup (Act# 13100-35488). The authors would like to acknowledge Mr. Don Marek from TAMUK—Department of Environmental Engineering; Ms. Maggie Ramirez and Mr. Daniel Diaz from TAMUK—Department of Biology and Health Sciences; Ms. Baimee Nguyen, Ms. Amber Chun, Ms. Roxana Apacible and Mr. Richard Wong from TAMHSC—Rangel College of Pharmacy; Mr. Shubham Manchanda from UT Dallas; and Mr. Vishal Sethi from Richard King High School for their technical help, software support, proof reading, literature search, and reference check.

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Authors and Affiliations

  1. Brigham & Women’s Hospital, Pain Management Center, Boston, MA, 02467, USA

    Rama Surya Prakash Perepu

  2. Division of Molecular Cardiology, Central Texas Veterans Administration Health Care System, Texas A&M University System Health Science Center, College Station, TX, 76504, USA

    David E. Dostal

  3. Department of Biological & Health Sciences, Texas A&M University Kingsville, Kingsville, TX, USA

    Carlos Garcia

  4. Loyola University Medical Center, Stritch School of Medicine, Maywood, IL, 60153, USA

    Richard H. Kennedy

  5. Cardiovascular Research and Development Laboratory, Texas A&M University System Health Science Center, Rangel College of Pharmacy, 1010 West Avenue B, MSC 131, Kingsville, TX, 78363-8202, USA

    Rajat Sethi

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  1. Rama Surya Prakash Perepu
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  2. David E. Dostal
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  3. Carlos Garcia
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  4. Richard H. Kennedy
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  5. Rajat Sethi
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Correspondence to Rajat Sethi.

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Perepu, R.S.P., Dostal, D.E., Garcia, C. et al. Cardiac dysfunction subsequent to chronic ozone exposure in rats. Mol Cell Biochem 360, 339–345 (2012). https://doi.org/10.1007/s11010-011-1073-1

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  • DOI: https://doi.org/10.1007/s11010-011-1073-1

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